論文 - 詳細
| RRC ID | 58460 |
|---|---|
| 著者 | Nakamigawa M, Kondo T, Maéno M. |
| タイトル | Specific activation of the hb4 gene in the Xenopus oocyte through a Nobox-binding element located at the proximal promoter sequence. |
| ジャーナル | Zygote |
| Abstract |
We isolated and characterized Xenopus tropicalis hb4 flanking DNA and showed that the -3076/+29 sequence was able to drive stage-specific transcription in the developmental process. Transgenic reporter analysis indicated that green fluorescent protein was expressed in the ovaries of female frogs at 3 months of age and in both the ovaries and testis of frogs at 6 months of age. A series of experiments with deletion of the flanking sequence and a subsequent luciferase reporter assay revealed that there were two positive regulatory regions and that the most proximal sequence of the promoter region had a certain level of transcriptional activity in oocytes. Subsequently, we showed that a conserved sequence containing Nobox-binding element (NBE) was essential for transcriptional activation and that Nobox expressed in the ovary had a crucial role in hb4 transcription through the NBE sequence. |
| 巻・号 | 27(4) |
| ページ | 195-202 |
| 公開日 | 2019-8-1 |
| DOI | 10.1017/S0967199419000017 |
| PII | S0967199419000017 |
| PMID | 31250783 |
| MeSH | Animals Animals, Genetically Modified Binding Sites / genetics Female Gene Expression Regulation, Developmental Histones / genetics Nuclear Proteins / genetics* Oocytes / metabolism* Ovary / cytology Ovary / metabolism Promoter Regions, Genetic / genetics* Regulatory Elements, Transcriptional / genetics* Transcriptional Activation Xenopus / genetics* Xenopus Proteins / genetics* Xenopus laevis / genetics |
| IF | 1.257 |
| 引用数 | 0 |
| リソース情報 | |
| ツメガエル・イモリ | |