RRC ID 58464
著者 Nakajima K, Tanizaki Y, Luu N, Zhang H, Shi YB.
タイトル Comprehensive RNA-Seq analysis of notochord-enriched genes induced during Xenopus tropicalis tail resorption.
ジャーナル Gen Comp Endocrinol
Abstract Anuran metamorphosis is perhaps the most dramatic developmental process regulated by thyroid hormone (TH). One of the unique processes that occur during metamorphosis is the complete resorption of the tail, including the notochord. Interestingly, recent gene knockout studies have shown that of the two known vertebrate TH receptors, TRα and TRβ, TRβ appears to be critical for notochord regression during tail resorption in Xenopus tropicalis. To determine the mechanisms underlying notochord regression, we carried out a comprehensive gene expression analysis in the notochord during metamorphosis by using RNA-Seq analyses of whole tail at stage 60 before any noticeable tail length reduction, whole tail at stage 63 when the tail length is reduced by about one half, and the rest of the tail at stage 63 after removing the notochord. This allowed us to identify many notochord-enriched, metamorphosis-induced genes at stage 63. Future studies on these genes should help to determine if they are regulated by TRβ and play any roles in notochord regression.
巻・号 287
ページ 113349
公開日 2020-2-1
DOI 10.1016/j.ygcen.2019.113349
PII S0016-6480(19)30405-8
PMID 31794731
PMC PMC6956247
MeSH Animals Gene Expression Regulation, Developmental / genetics* Notochord / growth & development* RNA-Seq / methods* Tail / growth & development* Xenopus / genetics* Xenopus laevis / growth & development*
IF 2.428
引用数 0
リソース情報
ツメガエル・イモリ