| RRC ID |
58473
|
| 著者 |
Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, Lynn K, Abe A, Kumaraswamy A, Tanimoto H.
|
| タイトル |
Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter receptors in individual neurons are therefore critical for understanding the operation of neural circuits. Here we describe a comprehensive library of reporter strains in which a convertible T2A-GAL4 cassette is inserted into endogenous neurotransmitter receptor genes of Drosophila. Using this library, we profile the expression of 75 neurotransmitter receptors in the brain. Cluster analysis reveals neurochemical segmentation of the brain, distinguishing higher brain centers from the rest. By recombinase-mediated cassette exchange, we convert T2A-GAL4 into split-GFP and Tango to visualize subcellular localization and activation of dopamine receptors in specific cell types. This reveals striking differences in their subcellular localization, which may underlie the distinct cellular responses to dopamine in different behavioral contexts. Our resources thus provide a versatile toolkit for dissecting the cellular organization and function of neurotransmitter systems in the fly brain.
|
| 巻・号 |
30(1)
|
| ページ |
284-297.e5
|
| 公開日 |
2020-1-7
|
| DOI |
10.1016/j.celrep.2019.12.018
|
| PII |
S2211-1247(19)31668-7
|
| PMID |
31914394
|
| MeSH |
Animals
Brain / diagnostic imaging*
CRISPR-Associated Protein 9 / metabolism
CRISPR-Cas Systems / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism*
Ethanol / adverse effects
Gene Expression Regulation
Genes, Reporter
Receptors, Dopamine / metabolism
Receptors, Neurotransmitter / metabolism*
|
| IF |
8.109
|
| 引用数 |
4
|
| リソース情報 |
| ショウジョウバエ |
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