論文 - 詳細
| RRC ID | 58473 |
|---|---|
| 著者 | Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, Lynn K, Abe A, Kumaraswamy A, Tanimoto H. |
| タイトル | Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors. |
| ジャーナル | Cell Rep |
| Abstract |
Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter receptors in individual neurons are therefore critical for understanding the operation of neural circuits. Here we describe a comprehensive library of reporter strains in which a convertible T2A-GAL4 cassette is inserted into endogenous neurotransmitter receptor genes of Drosophila. Using this library, we profile the expression of 75 neurotransmitter receptors in the brain. Cluster analysis reveals neurochemical segmentation of the brain, distinguishing higher brain centers from the rest. By recombinase-mediated cassette exchange, we convert T2A-GAL4 into split-GFP and Tango to visualize subcellular localization and activation of dopamine receptors in specific cell types. This reveals striking differences in their subcellular localization, which may underlie the distinct cellular responses to dopamine in different behavioral contexts. Our resources thus provide a versatile toolkit for dissecting the cellular organization and function of neurotransmitter systems in the fly brain. |
| 巻・号 | 30(1) |
| ページ | 284-297.e5 |
| 公開日 | 2020-1-7 |
| DOI | 10.1016/j.celrep.2019.12.018 |
| PII | S2211-1247(19)31668-7 |
| PMID | 31914394 |
| MeSH | Animals Brain / diagnostic imaging* CRISPR-Associated Protein 9 / metabolism CRISPR-Cas Systems / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Ethanol / adverse effects Gene Expression Regulation Genes, Reporter Receptors, Dopamine / metabolism Receptors, Neurotransmitter / metabolism* |
| IF | 8.109 |
| 引用数 | 4 |
| リソース情報 | |
| ショウジョウバエ | DGRC#119312 DGRC#119313 DGRC#119314 DGRC#119381 DGRC#119315 DGRC#119382 DGRC#119316 DGRC#119383 DGRC#119317 DGRC#119384 DGRC#119385 DGRC#119318 DGRC#119386 DGRC#119319 DGRC#119320 DGRC#119321 DGRC#119322 DGRC#119323 DGRC#119324 DGRC#119325 DGRC#119326 DGRC#119327 DGRC#119328 DGRC#119329 DGRC#119387 DGRC#119330 DGRC#119388 DGRC#119331 DGRC#119332 DGRC#119333 DGRC#119334 DGRC#119389 DGRC#119335 DGRC#119390 DGRC#119391 DGRC#119336 DGRC#119337 DGRC#119338 DGRC#119339 DGRC#119392 DGRC#119340 DGRC#119393 DGRC#119341 DGRC#119342 DGRC#119343 DGRC#119344 DGRC#119345 DGRC#119346 DGRC#119347 DGRC#119348 DGRC#119349 DGRC#119350 DGRC#119351 DGRC#119352 DGRC#119353 DGRC#119354 DGRC#119355 DGRC#119356 DGRC#119357 DGRC#119358 DGRC#119359 DGRC#119360 DGRC#119361 DGRC#119362 DGRC#119363 DGRC#119364 DGRC#119365 DGRC#119366 DGRC#119367 DGRC#119368 DGRC#119369 DGRC#119370 DGRC#119371 DGRC#119372 DGRC#119373 DGRC#119374 DGRC#119375 DGRC#119376 DGRC#119377 DGRC#119378 DGRC#119379 DGRC#119380 |