RRC ID 585
Author El-Din El-Assal S, Le J, Basu D, Mallery EL, Szymanski DB.
Title DISTORTED2 encodes an ARPC2 subunit of the putative Arabidopsis ARP2/3 complex.
Journal Plant J.
Abstract Arabidopsis trichomes are unicellular, branched structures that have highly constrained requirements for the cytoskeleton. The 'distorted group' genes function downstream from microtubule-based branch initiation, and are required during the actin-dependent phase of polarized stalk and branch expansion. Of the eight known 'distorted group' genes, a subset encode homologs of ARP2/3 complex subunits. In eukaryotic cells, the seven-protein ARP2/3 complex nucleates actin filament networks that push on the plasma membrane and organelles. In plants cells, the existence and function of an ARP2/3 complex is unclear. In this paper, we report that DISTORTED2 (DIS2) encodes a paralogue of the ARP2/3 complex subunit ARPC2. DIS2 has ARPC2 activity, based on its ability to rescue the growth defects of arpc2 (arc35Delta) null yeast cells. Like known ARPC2s, DIS2 physically interacts with ARPC4. Mutations in DIS2 cause a distorted trichome phenotype, defects in cell-cell adhesion, and a modest reduction in shoot FW. The actin cytoskeleton in dis2 trichomes is extensive, but developing branches fail to generate and maintain highly organized cytoplasmic actin bundles.
Volume 38(3)
Pages 526-38
Published 2004-5
DOI 10.1111/j.1365-313X.2004.02065.x
PMID 15086808
MeSH Actin-Related Protein 2 Actin-Related Protein 2-3 Complex Actin-Related Protein 3 Actins / genetics* Actins / metabolism Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Cell Surface Extensions / physiology Cloning, Molecular Microfilament Proteins / genetics* Microfilament Proteins / metabolism Molecular Sequence Data Sequence Homology, Amino Acid
IF 5.726
Arabidopsis / Cultured plant cells, genes pda11504