Reference - Detail
| RRC ID | 58549 |
|---|---|
| Author | Lu Z, Dai J, Zheng L, Teng Z, Zhang Q, Qiu D, Song L. |
| Title | Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus. |
| Journal | Bioresour Technol |
| Abstract |
Improving carbon availability in astaxanthin production is pivotal in Haematococcus industry. In this study, disodium 2-oxoglutarate (2-OG-2Na) was observed to be a potential carbon regulator to increase the astaxanthin content. To illustrate its efficacy in astaxanthin production, key genes and enzyme were analyzed. Upon 2-OG-2Na treatment, genes ipi, bkt and crtR-b were up regulated, concomitantly, carotenoids and astaxanthin content increased by 15.4% and 14.0% at 120 h, respectively; additionally, Acetyl-CoA carboxylase was activated, consistent with 1.27-fold increase in fatty acids content. PUFAs increased earlier as fatty acids assembly gene fad was up-regulated to 20.56. It was also found that cell division was not compromised. Altogether, it was suggested that increased carbon skeletons were re-directed into the astaxanthin and fatty acids biosynthesis pathway. Furthermore, 2-OG-2Na was applied in ten Haematococcus strains. Of these strains, astaxanthin contents were accelerated with average net increase of 10.48%, exhibiting a scalable paradigm for commercial production. |
| Volume | 299 |
| Pages | 122612 |
| Published | 2020-3-1 |
| DOI | 10.1016/j.biortech.2019.122612 |
| PII | S0960-8524(19)31842-5 |
| PMID | 31874452 |
| MeSH | Carbon Cycle Fatty Acids Ketoglutaric Acids* Xanthophylls* |
| IF | 7.539 |
| Times Cited | 0 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-144 |