| RRC ID |
58566
|
| 著者 |
Fukudome M, Watanabe E, Osuki KI, Imaizumi R, Aoki T, Becana M, Uchiumi T.
|
| タイトル |
Stably Transformed Lotus japonicus Plants Overexpressing Phytoglobin LjGlb1-1 Show Decreased Nitric Oxide Levels in Roots and Nodules as Well as Delayed Nodule Senescence.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
The class 1 phytoglobin, LjGlb1-1, is expressed in various tissues of the model legume Lotus japonicus, where it may play multiple functions by interacting with nitric oxide (NO). One of such functions is the onset of a proper symbiosis with Mesorhizobium loti resulting in the formation of actively N2-fixing nodules. Stable overexpression lines (Ox1 and Ox2) of LjGlb1-1 were generated and phenotyped. Both Ox lines showed reduced NO levels in roots and enhanced nitrogenase activity in mature and senescent nodules relative to the wild-type (WT). Physiological and cytological observations indicated that overexpression of LjGlb1-1 delayed nodule senescence. The application to WT nodules of the NO donor S-nitroso-N-acetyl-dl-penicillamine (SNAP) or the phytohormones abscisic acid (ABA) and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) repressed nitrogenase activity, induced the expression of three senescence-associated genes and caused cytological changes evidencing nodule senescence. These effects were almost completely reverted by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. Our results reveal that overexpression of LjGlb1-1 improves the activity of mature nodules and delays nodule senescence in the L.japonicus-M.loti symbiosis. These beneficial effects are probably mediated by the participation of LjGlb1-1 in controlling the concentration of NO that may be produced downstream in the phytohormone signaling pathway in nodules.
|
| 巻・号 |
60(4)
|
| ページ |
816-825
|
| 公開日 |
2018-12-28
|
| DOI |
10.1093/pcp/pcy245
|
| PII |
5265248
|
| PMID |
30597068
|
| MeSH |
Amino Acids, Cyclic / metabolism
Gene Expression Regulation, Plant
Hemoglobins / metabolism
Lotus / metabolism*
Nitric Oxide / metabolism*
Nitrogen Fixation / physiology
Plant Roots / metabolism
Root Nodules, Plant / metabolism
Root Nodules, Plant / physiology
|
| IF |
4.062
|
| 引用数 |
10
|
| リソース情報 |
| ミヤコグサ・ダイズ |
L.japonicus Gifu B-129 |
