RRC ID |
5861
|
著者 |
Ashida N, Ishii S, Hayano S, Tago K, Tsuji T, Yoshimura Y, Otsuka S, Senoo K.
|
タイトル |
Isolation of functional single cells from environments using a micromanipulator: application to study denitrifying bacteria.
|
ジャーナル |
Appl Microbiol Biotechnol
|
Abstract |
We developed a novel method to isolate functionally active single cells from environmental samples and named it the functional single-cell (FSC) isolation method. This method is based on a combination of substrate-responsive direct viable counts, live-cell staining with 5-carboxyfluorescein diacetate acetoxymethyl ester, and micromanipulation followed by cultivation in a medium. To evaluate this method, we applied it to study a denitrifying community in rice paddy soil. Similar denitrifier counts were obtained by the conventional most probable number analysis and our FSC isolation method. Using the FSC isolation method, 37 denitrifying bacteria were isolated, some of which harbored copper-containing nitrite reductase gene (nirK). The 16S rRNA gene analysis showed that members belonging to the genera Azospirillum and Ochrobactrum may be the major denitrifiers in the rice paddy soil. These results indicate that the FSC isolation method is a useful tool to obtain functionally active single cells from environmental samples.
|
巻・号 |
85(4)
|
ページ |
1211-7
|
公開日 |
2010-1-1
|
DOI |
10.1007/s00253-009-2330-z
|
PMID |
19936739
|
MeSH |
Azospirillum / classification
Azospirillum / isolation & purification
Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification*
Bacteriological Techniques*
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Fluoresceins
Nitrates / metabolism
Ochrobactrum / classification
Ochrobactrum / isolation & purification
Oryza*
Phylogeny
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
|
IF |
3.53
|
引用数 |
52
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 20522
JCM 1224
JCM 2505
JCM 21446
JCM 21315
JCM 12830
JCM 21032
JCM 20264
JCM 5963
JCM 5965 |