RRC ID 58615
著者 Hatano H, Goda T, Matsumoto A, Miyahara Y.
タイトル Induced Proton Dynamics on Semiconductor Surfaces for Sensing Tight Junction Formation Enhanced by an Extracellular Matrix and Drug.
ジャーナル ACS Sens
Abstract In the fields of tissue engineering and drug discovery, confirming the formation and maturation of epithelial cell tight junctions (TJs), which are necessary for blocking pathogenic invasion and absorption of nutrients and ions, at a high spatiotemporal resolution is essential. We previously developed a system of monitoring pH perturbation induced by weak acid exposure to cells cultured on an ion-sensitive field-effect transistor that enables a sensitive and specific detection of biomembrane injuries and TJ breakdowns caused by external stimuli such as nanomaterials and cytotoxins. In this study, we monitor time-lapse changes in the paracellular diffusion of growing epithelial cell monolayers using the pH perturbation assay as well as conventional permeability and trans-epithelial electrical resistance assays. The effects of the extracellular matrix and a TJ potentiator (KN-93) on epithelial TJ formation are evaluated. TJ formations were promoted on the substrate coated with Matrigel more than on the one coated with poly(l-lysine). KN-93 accelerated TJ formations in a dose-dependent manner. The pH perturbation assay denoted a longer incubation time for the completion of TJ formation compared with the conventional assays under the same conditions. Importantly, the pH perturbation assay is able to rigorously evaluate TJ formation, as the assay uses protons as the smallest indicator for detecting paracellular gaps, and the pH perturbation is specific to TJ alterations. These features for in vitro TJ evaluation using proton dynamics are advantageous for applications in tissue engineering and drug development.
巻・号 4(12)
ページ 3195-3202
公開日 2019-12-27
DOI 10.1021/acssensors.9b01635
PMID 31763825
MeSH Animals Benzylamines / pharmacology* Dogs Electrochemical Techniques / methods Extracellular Matrix / physiology Hydrogen-Ion Concentration Madin Darby Canine Kidney Cells Protons* Semiconductors* Sulfonamides / pharmacology* Tight Junctions / drug effects* Tight Junctions / physiology*
IF 6.944
引用数 0
リソース情報
ヒト・動物細胞 MDCK(RCB0995)