| RRC ID |
58633
|
| 著者 |
Hu Y, Domínguez CM, Bauer J, Weigel S, Schipperges A, Oelschlaeger C, Willenbacher N, Keppler S, Bastmeyer M, Heißler S, Wöll C, Scharnweber T, Rabe KS, Niemeyer CM.
|
| タイトル |
Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Biomedical applications require substrata that allow for the grafting, colonization and control of eukaryotic cells. Currently available materials are often limited by insufficient possibilities for the integration of biological functions and means for tuning the mechanical properties. We report on tailorable nanocomposite materials in which silica nanoparticles are interwoven with carbon nanotubes by DNA polymerization. The modular, well controllable and scalable synthesis yields materials whose composition can be gradually adjusted to produce synergistic, non-linear mechanical stiffness and viscosity properties. The materials were exploited as substrata that outperform conventional culture surfaces in the ability to control cellular adhesion, proliferation and transmigration through the hydrogel matrix. The composite materials also enable the construction of layered cell architectures, the expansion of embryonic stem cells by simplified cultivation methods and the on-demand release of uniformly sized stem cell spheroids.
|
| 巻・号 |
10(1)
|
| ページ |
5522
|
| 公開日 |
2019-12-4
|
| DOI |
10.1038/s41467-019-13381-1
|
| PII |
10.1038/s41467-019-13381-1
|
| PMID |
31797918
|
| PMC |
PMC6892801
|
| MeSH |
Cell Adhesion / drug effects
Cell Survival / drug effects
Coated Materials, Biocompatible / chemistry*
Coated Materials, Biocompatible / pharmacology
DNA / chemistry
DNA / genetics
DNA / metabolism
Humans
Hydrogels / chemistry
MCF-7 Cells
Materials Testing / methods
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanocomposites / chemistry*
Nanocomposites / ultrastructure
Nanotubes, Carbon / chemistry*
Silicon Dioxide / chemistry*
Tensile Strength
Viscosity
|
| IF |
12.121
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
EB5(AES0151) |
