論文 - 詳細
| RRC ID | 58640 |
|---|---|
| 著者 | Noguchi K, Hirano M, Hashimoto T, Yuba E, Takatani-Nakase T, Nakase I. |
| タイトル | Effects of Lyophilization of Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles on Intracellular Delivery. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Extracellular vesicles (exosomes, EVs) (30-200 nm in diameter) are secreted by various cells in the body. Owing to the pharmaceutical advantages of EVs, an EV-based drug delivery system (DDS) for cancer therapy is expected to be the next-generation therapeutic system. However, preservation methods for functional and therapeutic EVs should be developed. Here, we developed the method of lyophilization of arginine-rich cell penetrating peptide (CPP)-modified EVs and investigated the effects of lyophilization on the characteristics of EVs. MATERIALS AND METHODS:Particle size, structure, zeta-potential, and cellular uptake efficacy of the arginine-rich CPP-modified EVs were analyzed. The model protein saporin (SAP), having anti-cancer effects, was encapsulated inside the EVs to assess the cytosolic release of EV content after cellular uptake. RESULTS:Lyophilization of the EVs did not affect their particle size, structure, zeta-potential, and cellular uptake efficacy; however, the biological activity of the encapsulated SAP was inhibited by lyophilization. CONCLUSION:Lyophilization of EVs may affect SAP structures and/or reduce the cytosolic release efficacy of EV's content after cellular uptake and needs attention in EV-based DDSs. |
| 巻・号 | 39(12) |
| ページ | 6701-6709 |
| 公開日 | 2019-12-1 |
| DOI | 10.21873/anticanres.13885 |
| PII | 39/12/6701 |
| PMID | 31810935 |
| MeSH | Animals Arginine* CHO Cells Cell Survival Cell-Penetrating Peptides / administration & dosage Cell-Penetrating Peptides / chemistry Cell-Penetrating Peptides / pharmacokinetics* Cricetulus Extracellular Vesicles / metabolism* Freeze Drying Humans Particle Size Pharmaceutical Vehicles* Pinocytosis Preservation, Biological / methods Saporins / administration & dosage Saporins / pharmacokinetics* Tetraspanin 30 / metabolism |
| IF | 1.994 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |