RRC ID 58640
Author Noguchi K, Hirano M, Hashimoto T, Yuba E, Takatani-Nakase T, Nakase I.
Title Effects of Lyophilization of Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles on Intracellular Delivery.
Journal Anticancer Res
Abstract BACKGROUND/AIM:Extracellular vesicles (exosomes, EVs) (30-200 nm in diameter) are secreted by various cells in the body. Owing to the pharmaceutical advantages of EVs, an EV-based drug delivery system (DDS) for cancer therapy is expected to be the next-generation therapeutic system. However, preservation methods for functional and therapeutic EVs should be developed. Here, we developed the method of lyophilization of arginine-rich cell penetrating peptide (CPP)-modified EVs and investigated the effects of lyophilization on the characteristics of EVs.
MATERIALS AND METHODS:Particle size, structure, zeta-potential, and cellular uptake efficacy of the arginine-rich CPP-modified EVs were analyzed. The model protein saporin (SAP), having anti-cancer effects, was encapsulated inside the EVs to assess the cytosolic release of EV content after cellular uptake.
RESULTS:Lyophilization of the EVs did not affect their particle size, structure, zeta-potential, and cellular uptake efficacy; however, the biological activity of the encapsulated SAP was inhibited by lyophilization.
CONCLUSION:Lyophilization of EVs may affect SAP structures and/or reduce the cytosolic release efficacy of EV's content after cellular uptake and needs attention in EV-based DDSs.
Volume 39(12)
Pages 6701-6709
Published 2019-12
DOI 10.21873/anticanres.13885
PII 39/12/6701
PMID 31810935
MeSH Animals Arginine* CHO Cells Cell Survival Cell-Penetrating Peptides / administration & dosage Cell-Penetrating Peptides / chemistry Cell-Penetrating Peptides / pharmacokinetics* Cricetulus Extracellular Vesicles / metabolism* Freeze Drying Humans Particle Size Pharmaceutical Vehicles* Pinocytosis Preservation, Biological / methods Saporins / administration & dosage Saporins / pharmacokinetics* Tetraspanin 30 / metabolism
IF 1.935
Times Cited 1
Human and Animal Cells CHO-K1(RCB0285)