| RRC ID |
58690
|
| Author |
Wu Y, Zhou Y, Huang CJ, Kobayashi H, Yan S, Ozeki Y, Wu Y, Sun CW, Yasumoto A, Yatomi Y, Lei C, Goda K.
|
| Title |
Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging.
|
| Journal |
Opt Express
|
| Abstract |
Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.
|
| Volume |
28(1)
|
| Pages |
519-532
|
| Published |
2020-1-6
|
| DOI |
10.1364/OE.380679
|
| PII |
425575
|
| PMID |
32118978
|
| MeSH |
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use
HL-60 Cells
Humans
Imaging, Three-Dimensional*
K562 Cells
Leukemia / drug therapy
Leukemia / pathology
Microfluidics*
Optics and Photonics*
Time Factors
|
| IF |
3.669
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
HL-60(RCB3683), K562 |
