Reference - Detail
| RRC ID | 586 |
|---|---|
| Author | Pedersen L, Henriksen A. |
| Title | Expression, purification and crystallization of two peroxisomal acyl-CoA oxidases from Arabidopsis thaliana. |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Abstract |
Two members of the acyl-CoA oxidase family from Arabidopsis thaliana have been cloned, overexpressed, purified and crystallized. Long-chain-specific acyl-CoA oxidase 1 crystals are characterized by a large variation in diffraction quality and non-isomorphous unit-cell parameters. The best crystals diffract to 2.0 angstrom using synchrotron radiation, have unit-cell parameters a = 85.2, b = 117.0, c = 131.0 angstrom, alpha = beta = gamma = 90 degrees and show P2(1)2(1)2(1) symmetry. There are two polypeptide chains in the asymmetric unit. Short-chain-specific acyl-CoA oxidase 4 crystals are trigonal, space group P3(1)21/P3(2)21, with unit-cell parameters a = b = 198.7, c = 149.6 angstroms. The crystals are most likely to contain six polypeptide chains in the asymmetric unit. Freshly prepared acyl-CoA oxidase 4 crystals diffract to 3.9 angstroms at cryogenic temperature at beamline I711, Max-Lab, but the diffraction quality degenerates after storage for only a few days in the crystallization drop. A selenomethionine-substituted form of the protein was produced and two-wavelength MAD data were collected at beamline BW7A, EMBL Outstation, Hamburg. |
| Volume | 60(Pt 6) |
| Pages | 1125-8 |
| Published | 2004-6-1 |
| DOI | 10.1107/S0907444904007577 |
| PII | S0907444904007577 |
| PMID | 15159576 |
| MeSH | Acyl-CoA Oxidase / chemistry* Acyl-CoA Oxidase / genetics Arabidopsis / enzymology* Arabidopsis Proteins / chemistry* Arabidopsis Proteins / genetics Cloning, Molecular Crystallography, X-Ray / methods* Genes, Plant Models, Chemical Peptides / chemistry Peroxisomes / enzymology* Plasmids / metabolism Synchrotrons Temperature Time Factors X-Ray Diffraction |
| IF | 5.266 |
| Times Cited | 6 |
| WOS Category | CRYSTALLOGRAPHY BIOCHEMICAL RESEARCH METHODS BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda05283 |