論文 - 詳細
| RRC ID | 58704 |
|---|---|
| 著者 | Kaga N, Akasaka T, Matsuura T, Yokoyama A, Yoshida Y. |
| タイトル | Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces. |
| ジャーナル | Biomed Mater Eng |
| Abstract |
BACKGROUND:Surface nanostructures in titanium (Ti) oral implants are critical for rapid osseointegration. OBJECTIVE:The purpose of this study was to evaluate the growth of osteoblast-like (Saos-2) and epithelial-like (Ca9-22) cells on nanopatterned Ti films. METHODS:Ti films with 500 nm grooves and pillars were fabricated by nanoimprinting, and seeded with Saos-2 and Ca9-22 cells. Cell viability and morphology were assessed by cell proliferation assay and scanning electron microscopy, respectively. RESULTS:As assessed after 1 hour, proliferation of Saos-2 cells was most robust on grooved films than on pillared and smooth films, in this order. These cells approximately doubled on grooved and pillared substrates in 24 hours and after 5 days, but not on smooth surfaces. In contrast, Ca9-22 cells favored smooth surfaces, followed by grooved and pillared films. Indeed, cells sparsely adhered to pillared films over 5 days of incubation (p < 0.05). CONCLUSIONS:The data show that Saos-2 and Ca9-22 cells respond differently to different nanostructures, and highlight the potential use of nanopatterns to promote bone regeneration or to prevent epithelial downgrowth at the implant-bone interface. |
| 巻・号 | 30(5-6) |
| ページ | 559-567 |
| 公開日 | 2020-1-1 |
| DOI | 10.3233/BME-191074 |
| PII | BME191074 |
| PMID | 31929132 |
| MeSH | Bone-Implant Interface / physiology Cell Adhesion / drug effects Cell Adhesion / physiology* Cell Line Cell Proliferation / drug effects Cell Proliferation / physiology* Coated Materials, Biocompatible / chemical synthesis Coated Materials, Biocompatible / chemistry* Coated Materials, Biocompatible / pharmacology Dental Implants* Epithelial Cells / drug effects Epithelial Cells / physiology Humans Materials Testing Microtechnology Osseointegration / drug effects Osseointegration / physiology* Osteoblasts / drug effects Osteoblasts / physiology Surface Properties Tissue Scaffolds / chemistry Titanium / chemistry* |
| IF | 1.243 |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB3688) Ca9-22 cells(RCB1976) |