Reference - Detail
| RRC ID | 58711 |
|---|---|
| Author | Minamizaki T, Nakao Y, Irie Y, Ahmed F, Itoh S, Sarmin N, Yoshioka H, Nobukiyo A, Fujimoto C, Niida S, Sotomaru Y, Tanimoto K, Kozai K, Sugiyama T, Bonnelye E, Takei Y, Yoshiko Y. |
| Title | The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice. |
| Journal | Commun Biol |
| Abstract |
Communication between osteoblasts and osteoclasts plays a key role in bone metabolism. We describe here an unexpected role for matrix vesicles (MVs), which bud from bone-forming osteoblasts and have a well-established role in initiation of bone mineralization, in osteoclastogenesis. We show that the MV cargo miR-125b accumulates in the bone matrix, with increased accumulation in transgenic (Tg) mice overexpressing miR-125b in osteoblasts. Bone formation and osteoblasts in Tg mice are normal, but the number of bone-resorbing osteoclasts is reduced, leading to higher trabecular bone mass. miR-125b in the bone matrix targets and degrades Prdm1, a transcriptional repressor of anti-osteoclastogenic factors, in osteoclast precursors. Overexpressing miR-125b in osteoblasts abrogates bone loss in different mouse models. Our results show that the MV cargo miR-125b is a regulatory element of osteoblast-osteoclast communication, and that bone matrix provides extracellular storage of miR-125b that is functionally active in bone resorption. |
| Volume | 3(1) |
| Pages | 30 |
| Published | 2020-1-16 |
| DOI | 10.1038/s42003-020-0754-2 |
| PII | 10.1038/s42003-020-0754-2 |
| PMID | 31949279 |
| PMC | PMC6965124 |
| MeSH | Animals Biological Transport Biomarkers Bone Matrix / metabolism* Bone Resorption / genetics* Bone Resorption / metabolism* Bone Resorption / pathology Cell Communication Extracellular Vesicles / metabolism* Gene Expression Regulation Immunohistochemistry Mice Mice, Transgenic MicroRNAs / genetics* Osteoblasts / metabolism Osteoclasts / metabolism Osteogenesis / genetics Positive Regulatory Domain I-Binding Factor 1 / genetics RNA Interference Signal Transduction |
| IF | 4.165 |
| Times Cited | 0 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) ST2(RCB0224) |