Reference - Detail
| RRC ID | 58716 |
|---|---|
| Author | Nakayama Y, Inoue E, Kato A, Iwai Y, Takai-Yamazaki M, Tsuruya Y, Yamaguchi A, Noda K, Nomoto T, Ganss B, Ogata Y. |
| Title | Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth. |
| Journal | Odontology |
| Abstract |
Follicular dendritic cell-secreted protein (FDC-SP) is secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium (JE). Its expression could be controlled during inflammatory process of gingiva; however, responsible mechanism for gingival overgrowth and involvement of FDC-SP in clinical condition is still unclear. We hypothesized that JE-specific genes are associated with the initiation of drug-induced gingival enlargement (DIGE) called gingival overgrowth, and investigated the changes of JE-specific gene's expression and their localization in overgrown gingiva from the patients. Immunohistochemical analysis revealed that the FDC-SP localization was spread in overgrown gingival tissues. FDC-SP mRNA levels in GE1 and Ca9-22 cells were increased by time-dependent nifedipine treatments, similar to other JE-specific genes, such as Amelotin (Amtn) and Lamininβ3 subunit (Lamβ3), whereas type 4 collagen (Col4) mRNA levels were decreased. Immunocytochemical analysis showed that FDC-SP, AMTN, and Lamβ3 protein levels were increased in GE1 and Ca9-22 cells. Transient transfection analyses were performed using luciferase constructs including various lengths of human FDC-SP gene promoter, nifedipine increased luciferase activities of -345 and -948FDC-SP constructs. These results raise the possibility that the nifedipine-induced FDC-SP may be related to the mechanism responsible for gingival overgrowth does not occur at edentulous jaw ridges. |
| Volume | 108(4) |
| Pages | 532-544 |
| Published | 2020-10-1 |
| DOI | 10.1007/s10266-020-00483-2 |
| PII | 10.1007/s10266-020-00483-2 |
| PMID | 31955298 |
| MeSH | Dendritic Cells, Follicular* Epithelial Attachment Gingiva Gingival Overgrowth* Humans Nifedipine |
| IF | 1.84 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | GE1(RCB1709) |