| RRC ID |
58716
|
| Author |
Nakayama Y, Inoue E, Kato A, Iwai Y, Takai-Yamazaki M, Tsuruya Y, Yamaguchi A, Noda K, Nomoto T, Ganss B, Ogata Y.
|
| Title |
Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth.
|
| Journal |
Odontology
|
| Abstract |
Follicular dendritic cell-secreted protein (FDC-SP) is secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium (JE). Its expression could be controlled during inflammatory process of gingiva; however, responsible mechanism for gingival overgrowth and involvement of FDC-SP in clinical condition is still unclear. We hypothesized that JE-specific genes are associated with the initiation of drug-induced gingival enlargement (DIGE) called gingival overgrowth, and investigated the changes of JE-specific gene's expression and their localization in overgrown gingiva from the patients. Immunohistochemical analysis revealed that the FDC-SP localization was spread in overgrown gingival tissues. FDC-SP mRNA levels in GE1 and Ca9-22 cells were increased by time-dependent nifedipine treatments, similar to other JE-specific genes, such as Amelotin (Amtn) and Lamininβ3 subunit (Lamβ3), whereas type 4 collagen (Col4) mRNA levels were decreased. Immunocytochemical analysis showed that FDC-SP, AMTN, and Lamβ3 protein levels were increased in GE1 and Ca9-22 cells. Transient transfection analyses were performed using luciferase constructs including various lengths of human FDC-SP gene promoter, nifedipine increased luciferase activities of -345 and -948FDC-SP constructs. These results raise the possibility that the nifedipine-induced FDC-SP may be related to the mechanism responsible for gingival overgrowth does not occur at edentulous jaw ridges.
|
| Volume |
108(4)
|
| Pages |
532-544
|
| Published |
2020-10-1
|
| DOI |
10.1007/s10266-020-00483-2
|
| PII |
10.1007/s10266-020-00483-2
|
| PMID |
31955298
|
| MeSH |
Dendritic Cells, Follicular*
Epithelial Attachment
Gingiva
Gingival Overgrowth*
Humans
Nifedipine
|
| IF |
1.84
|
| Resource |
| Human and Animal Cells |
GE1(RCB1709) |
