| RRC ID |
58745
|
| 著者 |
Kitao A, Kawamoto S, Kurata K, Hayakawa I, Yamasaki T, Matsuoka H, Sumi Y, Kakeji Y, Kamesaki T, Minami H.
|
| タイトル |
Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.
|
| ジャーナル |
Int J Hematol
|
| Abstract |
Autoimmune hemolytic anemia (AIHA) is a rare comorbidity in colorectal cancer (CRC) and has an unknown etiology. Previously, we described an AIHA case secondary to CRC with ectopic band 3 expression. Herein, we investigated ectopic band 3 expression and erythrocyte membrane-bound IgG in a CRC cohort. Between September 2016 and August 2018, 50 patients with CRC and 26 healthy controls were enrolled in the present study. The expression of band 3 and SLC4A1 mRNA was observed in 97% of CRC surgical specimens. Although clinical AIHA was not observed in any patient with CRC, a direct antiglobulin test was positive in 10 of the patients in the CRC group (p = 0.01). Flow cytometry revealed significantly increased erythrocyte membrane-bound IgG among patients with CRC compared to healthy controls (mean ± standard deviation; 38.8 ± 4.7 vs. 29.9 ± 15.6, p = 0.012). Normocytic anemia was observed, including in cases negative for fecal occult blood, suggesting a shortened erythrocyte life-span due to increased membrane-bound IgG. Immunoprecipitation revealed increased anti-band 3 autoantibodies in patients' sera. Mouse experiments recapitulated this phenomenon. We also confirmed that band 3 expression is controlled by 5'AMP-activated protein kinase under hypoxic conditions. These findings increase our understanding of the etiology of cancer-related anemia.
|
| 巻・号 |
111(5)
|
| ページ |
657-666
|
| 公開日 |
2020-5-1
|
| DOI |
10.1007/s12185-020-02831-y
|
| PII |
10.1007/s12185-020-02831-y
|
| PMID |
31997080
|
| MeSH |
Anemia / etiology*
Anemia / immunology
Animals
Anion Exchange Protein 1, Erythrocyte / genetics*
Anion Exchange Protein 1, Erythrocyte / metabolism*
Colorectal Neoplasms / complications*
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / immunology
Colorectal Neoplasms / metabolism
Erythrocyte Membrane / immunology*
Gene Expression*
Humans
Immunoglobulin G / immunology*
Immunoglobulin G / metabolism*
|
| IF |
2.245
|
| リソース情報 |
| ヒト・動物細胞 |
WEHI-3(RCB0035) |
