| RRC ID |
58762
|
| 著者 |
Hirano S, Etoh T, Okunaga R, Shibata K, Ohta M, Nishizono A, Kitano S.
|
| タイトル |
Reovirus inhibits the peritoneal dissemination of pancreatic cancer cells in an immunocompetent animal model.
|
| ジャーナル |
Oncol Rep
|
| Abstract |
The prognosis of pancreatic cancer with peritoneal dissemination has not improved. The aim of this study was to clarify whether oncolytic reovirus is effective against the peritoneal dissemination of pancreatic cancer in an immunocompetent animal model. The hamster pancreatic cancer cells HaP-T1 were inoculated into the peritoneal cavity of the hamster and reovirus (1x10(8) plaque-forming units) was administered into the peritoneal cavity on days 1, 3, 5 and 7 after HaP-T1 inoculations. The number and weight of the disseminated nodules in each group were recorded. Reovirus protein in the disseminated nodules was examined by immunohistochemical staining. The tumor volumes of peritoneal dissemination in the treatment group were significantly less than those in the control group (p<0.05). In addition, the amount of ascites was decreased in the treatment group in comparison to the control group. Immunohistochemical examination revealed that reovirus replication was seen only in the disseminated nodules but not in surrounding normal tissues. There were no serious side effects observed in this study. These data suggested that intraperitoneal administration of reovirus might be an effective form of oncolytic viral therapy for peritoneal dissemination of pancreatic cancer.
|
| 巻・号 |
21(6)
|
| ページ |
1381-4
|
| 公開日 |
2009-6-1
|
| DOI |
10.3892/or_00000364
|
| PMID |
19424613
|
| MeSH |
Animals
Ascites / pathology
Ascites / prevention & control
Ascites / virology
Cell Line, Tumor
Cricetinae
Immunocompetence
Injections, Intraperitoneal
Male
Mammalian orthoreovirus 3 / pathogenicity*
Mesocricetus
Neoplasms, Experimental
Oncolytic Virotherapy*
Pancreatic Neoplasms / pathology
Pancreatic Neoplasms / therapy*
Pancreatic Neoplasms / virology
Peritoneal Neoplasms / prevention & control*
Peritoneal Neoplasms / secondary
Peritoneal Neoplasms / virology
Time Factors
|
| リソース情報 |
| ヒト・動物細胞 |
HaP-T1(RCB0411) |
