| RRC ID |
58763
|
| 著者 |
Funakoshi-Tago M, Nonaka Y, Tago K, Takeda M, Ishihara Y, Sakai A, Matsutaka M, Kobata K, Tamura H.
|
| タイトル |
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Coffee is a complex mixture of many bioactive compounds possessing anti-inflammatory properties. However, the mechanisms by which coffee exerts anti-inflammatory effects remains unclear and the active ingredients have not yet been identified. In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that anti-inflammatory activity of coffee required the roasting process. Interestingly, we identified pyrocatechol, a degradation product derived from chlorogenic acid during roasting, as the active ingredient exhibiting anti-inflammatory activity in coffee. HPLC analysis showed that 124 μM pyrocatechol was included in 100% (v/v) roasted coffee. A treatment with 5%(v/v) coffee extract and more than 2.5 μM pyrocatechol inhibited the LPS-induced activation of NF-κB and also significantly activated Nrf2, which acts as a negative regulator in LPS-induced inflammation. Furthermore, intake of 60% (v/v) coffee extract and 74.4 μM pyrocatechol, which is the concentration equal to contained in 60% (v/v) coffee, markedly inhibited the LPS-induced inflammatory responses in mice. Collectively, these results demonstrated that pyrocatechol, which was formed by the roasting of coffee green beans, is one of the ingredients contributing to the anti-inflammatory activity of coffee.
|
| 巻・号 |
10(1)
|
| ページ |
2584
|
| 公開日 |
2020-2-13
|
| DOI |
10.1038/s41598-020-59380-x
|
| PII |
10.1038/s41598-020-59380-x
|
| PMID |
32054966
|
| PMC |
PMC7018815
|
| MeSH |
Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / pharmacology*
Catechols / chemistry
Catechols / pharmacology*
Coffee / chemistry*
Inflammation / drug therapy
Inflammation / immunology
Lipopolysaccharides / immunology*
Mice
NF-E2-Related Factor 2 / immunology*
NF-kappa B / antagonists & inhibitors*
NF-kappa B / immunology
RAW 264.7 Cells
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
