論文 - 詳細
| RRC ID | 58763 |
|---|---|
| 著者 | Funakoshi-Tago M, Nonaka Y, Tago K, Takeda M, Ishihara Y, Sakai A, Matsutaka M, Kobata K, Tamura H. |
| タイトル | Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2. |
| ジャーナル | Sci Rep |
| Abstract |
Coffee is a complex mixture of many bioactive compounds possessing anti-inflammatory properties. However, the mechanisms by which coffee exerts anti-inflammatory effects remains unclear and the active ingredients have not yet been identified. In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that anti-inflammatory activity of coffee required the roasting process. Interestingly, we identified pyrocatechol, a degradation product derived from chlorogenic acid during roasting, as the active ingredient exhibiting anti-inflammatory activity in coffee. HPLC analysis showed that 124 μM pyrocatechol was included in 100% (v/v) roasted coffee. A treatment with 5%(v/v) coffee extract and more than 2.5 μM pyrocatechol inhibited the LPS-induced activation of NF-κB and also significantly activated Nrf2, which acts as a negative regulator in LPS-induced inflammation. Furthermore, intake of 60% (v/v) coffee extract and 74.4 μM pyrocatechol, which is the concentration equal to contained in 60% (v/v) coffee, markedly inhibited the LPS-induced inflammatory responses in mice. Collectively, these results demonstrated that pyrocatechol, which was formed by the roasting of coffee green beans, is one of the ingredients contributing to the anti-inflammatory activity of coffee. |
| 巻・号 | 10(1) |
| ページ | 2584 |
| 公開日 | 2020-2-13 |
| DOI | 10.1038/s41598-020-59380-x |
| PII | 10.1038/s41598-020-59380-x |
| PMID | 32054966 |
| PMC | PMC7018815 |
| MeSH | Animals Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / pharmacology* Catechols / chemistry Catechols / pharmacology* Coffee / chemistry* Inflammation / drug therapy Inflammation / immunology Lipopolysaccharides / immunology* Mice NF-E2-Related Factor 2 / immunology* NF-kappa B / antagonists & inhibitors* NF-kappa B / immunology RAW 264.7 Cells |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |