RRC ID 58772
Author Ideo H, Kondo J, Nomura T, Nonomura N, Inoue M, Amano J.
Title Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.
Journal Sci Rep
Abstract Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer. Understanding the structure of cancer-specific glycans can help us improve PSA assay. In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA. The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry. PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1. Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA. Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans. Furthermore, the existence of Lewis X antigen group on PSA was indicated. These PSAs will be candidates for new cancer-related markers.
Volume 10(1)
Pages 2708
Published 2020-2-17
DOI 10.1038/s41598-020-59622-y
PII 10.1038/s41598-020-59622-y
PMID 32066783
PMC PMC7026178
MeSH Biomarkers, Tumor / chemistry Biomarkers, Tumor / metabolism* Carbohydrate Sequence Cell Line, Tumor Chromatography, Affinity Concanavalin A / chemistry Culture Media, Conditioned / chemistry Glycopeptides / chemistry Glycopeptides / metabolism Glycosylation Humans Lewis X Antigen / chemistry Lewis X Antigen / metabolism Male Plant Lectins / chemistry Polysaccharides / chemistry* Polysaccharides / isolation & purification Polysaccharides / metabolism Prostate / metabolism Prostate / pathology Prostate-Specific Antigen / chemistry Prostate-Specific Antigen / metabolism* Prostatic Neoplasms / diagnosis* Prostatic Neoplasms / metabolism Prostatic Neoplasms / pathology Protein Processing, Post-Translational* Receptors, N-Acetylglucosamine / chemistry Semen / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spheroids, Cellular / chemistry Spheroids, Cellular / metabolism* Spheroids, Cellular / pathology
IF 3.998
Human and Animal Cells LNCap.FGC(RCB2144)