論文 - 詳細
| RRC ID | 58840 |
|---|---|
| 著者 | Uchikawa M, Kato M, Nagata A, Sanada S, Yoshikawa Y, Tsunematsu Y, Sato M, Suzuki T, Hashidume T, Watanabe K, Yoshikawa Y, Miyoshi N. |
| タイトル | Elevated levels of proinflammatory volatile metabolites in feces of high fat diet fed KK-Ay mice. |
| ジャーナル | Sci Rep |
| Abstract |
When the microfloral composition deteriorates, it triggers low-level chronic inflammation associated with several lifestyle-related diseases including obesity and diabetic mellitus. Fecal volatile organic compounds (VOCs) have been found to differ in gastrointestinal diseases as well as intestinal infection. In this study, to evaluate a potential association between the pathogenesis of lifestyle-related diseases and VOCs in the intestinal tract, fecal VOCs from obese/diabetic KK-Ay mice (KK) or controls (C57BL/6J mice; BL) fed a normal or high fat diet (NFD or HFD) were investigated using headspace sampler-GC-EI-MS. Principal component analysis (PCA) of fecal VOC profiles clearly separated the experimental groups depending on the mouse lineage (KK vs BL) and the diet type (NFD vs HFD). 16 s rRNA sequencing revealed that the PCA distribution of VOCs was in parallel with the microfloral composition. We identified that some volatile metabolites including n-alkanals (nonanal and octanal), acetone and phenol were significantly increased in the HFD and/or KK groups. Additionally, these volatile metabolites induced proinflammatory activity in the RAW264 murine macrophage cell line indicating these bioactive metabolites might trigger low-level chronic inflammation. These results suggest that proinflammatory VOCs detected in HFD-fed and/or diabetic model mice might be novel noninvasive diagnosis biomarkers for diabetes. |
| 巻・号 | 10(1) |
| ページ | 5681 |
| 公開日 | 2020-3-30 |
| DOI | 10.1038/s41598-020-62541-7 |
| PII | 10.1038/s41598-020-62541-7 |
| PMID | 32231228 |
| PMC | PMC7105489 |
| MeSH | Animals Biomarkers / analysis Diet, High-Fat / adverse effects* Disease Models, Animal Feces / chemistry Gastrointestinal Microbiome / genetics Inflammation Male Mice Mice, Inbred C57BL Mice, Obese Obesity / metabolism* RAW 264.7 Cells RNA, Ribosomal, 16S / genetics Volatile Organic Compounds / analysis* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |