RRC ID |
58928
|
Author |
Schreiber M, Mascher M, Wright J, Padmarasu S, Himmelbach A, Heavens D, Milne L, Clavijo BJ, Stein N, Waugh R.
|
Title |
A Genome Assembly of the Barley 'Transformation Reference' Cultivar Golden Promise.
|
Journal |
G3 (Bethesda)
|
Abstract |
Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.
|
Volume |
10(6)
|
Pages |
1823-1827
|
Published |
2020-4-2
|
DOI |
10.1534/g3.119.401010
|
PII |
g3.119.401010
|
PMID |
32241919
|
PMC |
PMC7263683
|
MeSH |
Genome
Genomics
Genotype
High-Throughput Nucleotide Sequencing
Hordeum* / genetics
|
IF |
2.781
|
Times Cited |
2
|
Resource |
Barley |
Full lengh cDNA sequences |