RRC ID 58935
Author Melonek J, Zhou R, Bayer PE, Edwards D, Stein N, Small I.
Title High intraspecific diversity of Restorer-of-fertility-like genes in barley.
Journal Plant J
Abstract Nuclear restorer of fertility (Rf) genes suppress the effects of mitochondrial genes causing cytoplasmic male sterility (CMS), a condition in which plants fail to produce viable pollen. Rf genes, many of which encode RNA-binding pentatricopeptide repeat (PPR) proteins, are applied in hybrid breeding to overcome CMS used to block self-pollination of the seed parent. Here, we characterise the repertoire of restorer-of-fertility-like (RFL) PPR genes in barley (Hordeum vulgare). We found 26 RFL genes in the reference genome ('Morex') and an additional 51 putative orthogroups (POGs) in a re-sequencing data set from 262 barley genotypes and landraces. Whereas the sequences of some POGs are highly conserved across hundreds of barley accessions, the sequences of others are much more variable. High sequence variation strongly correlates with genomic location - the most variable genes are found in a cluster on chromosome 1H. A much higher likelihood of diversifying selection was found for genes within this cluster than for genes present as singlets. This work includes a comprehensive analysis of the patterns of intraspecific variation of RFL genes. The RFL sequences characterised in this study will be useful for the development of new markers for fertility restoration loci.
Volume 97(2)
Pages 281-295
Published 2019-1-1
DOI 10.1111/tpj.14115
PMID 30276910
PMC PMC7380019
MeSH Crops, Agricultural Genetic Variation* Hordeum / genetics* Multigene Family* Plant Breeding Plant Infertility / genetics* Plant Proteins / genetics Plant Proteins / metabolism RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Species Specificity
IF 6.141
Times Cited 5
Barley Barley accession Haruna Nijo