論文 - 詳細
| RRC ID | 58939 |
|---|---|
| 著者 | Susaki EA, Shimizu C, Kuno A, Tainaka K, Li X, Nishi K, Morishima K, Ono H, Ode KL, Saeki Y, Miyamichi K, Isa K, Yokoyama C, Kitaura H, Ikemura M, Ushiku T, Shimizu Y, Saito T, Saido TC, Fukayama M, Onoe H, Touhara K, Isa T, Kakita A, Shibayama M, Ueda HR. |
| タイトル | Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues. |
| ジャーナル | Nat Commun |
| Abstract |
Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of the staining system, we developed a highly optimized 3D staining imaging pipeline based on CUBIC. Based on our precise characterization of biological tissues as an electrolyte gel, we experimentally evaluated broad 3D staining conditions by using an artificial tissue-mimicking material. The combination of optimized conditions allows a bottom-up design of a superior 3D staining protocol that can uniformly label whole adult mouse brains, an adult marmoset brain hemisphere, an ~1 cm3 tissue block of a postmortem adult human cerebellum, and an entire infant marmoset body with dozens of antibodies and cell-impermeant nuclear stains. The whole-organ 3D images collected by light-sheet microscopy are used for computational analyses and whole-organ comparison analysis between species. This pipeline, named CUBIC-HistoVIsion, thus offers advanced opportunities for organ- and organism-scale histological analysis of multicellular systems. |
| 巻・号 | 11(1) |
| ページ | 1982 |
| 公開日 | 2020-4-27 |
| DOI | 10.1038/s41467-020-15906-5 |
| PII | 10.1038/s41467-020-15906-5 |
| PMID | 32341345 |
| PMC | PMC7184626 |
| MeSH | Adult Animals Animals, Newborn Brain / pathology* Callithrix Cerebellum / pathology* Electrolytes* Female Fluorescent Dyes Humans Image Processing, Computer-Assisted Imaging, Three-Dimensional* Male Mice Mice, Inbred C57BL Microscopy, Fluorescence* Optical Imaging |
| IF | 12.121 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 427 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06334 |