RRC ID 58982
Author Arai T, Majima H, Watanabe A, Kamei K.
Title A Simple Method To Detect Point Mutations in Aspergillus fumigatus cyp51A Gene Using a Surveyor Nuclease Assay.
Journal Antimicrob Agents Chemother
Abstract One of the main mechanisms of azole resistance of Aspergillus fumigatus is thought to be a reduction in the drug's affinity for the target molecule, Cyp51A, due to its amino acid mutation(s). It is known that the azole resistance pattern is closely related to the mutation site(s) of the molecule. In this study, we tried to develop a simple and rapid detection method for cyp51A mutations using the endonuclease Surveyor nuclease. The Surveyor nuclease assay was verified using several azole-resistant strains of A. fumigatus that possess point mutations in Cyp51A. For validation of the Surveyor nuclease assay, blind tests were conducted using 48 strains of A. fumigatus (17 azole-resistant and 31 azole-susceptible strains). The Surveyor nuclease assay could rapidly detect cyp51A mutations with one primer set. Also, all the tested strains harboring different cyp51A single point mutations could be clearly distinguished from the wild type. The Surveyor nuclease assay is a simple method that can detect cyp51A mutations rapidly.
Volume 64(4)
Published 2020-3-24
DOI 10.1128/AAC.02271-19
PII AAC.02271-19
PMID 32015034
PMC PMC7179262
MeSH Aspergillus fumigatus / genetics* Cytochrome P-450 Enzyme System / genetics* DNA Mutational Analysis / methods* Drug Resistance, Fungal / genetics Endonucleases / genetics Fungal Proteins / genetics* Point Mutation* Reproducibility of Results
IF 4.904
Times Cited 0
Resource
Pathogenic eukaryotic microorganisms IFM 60516 IFM 58402 IFM 51977 IFM 60065 IFM 61960 IFM 51748 IFM 62821 IFM 64259 IFM 63666 IFM 63224 IFM 64304 IFM 62520 IFM 60237 IFM 50999 IFM 50268 IFM 55548 IFM 63488 IFM 62105 IFM 63311 IFM 63355 IFM 60901 IFM 62674 IFM 62709 IFM 52659 IFM 62104 IFM 63432 IFM 57130 IFM 60814 IFM 64460 IFM 49435 IFM 63345 IFM 62871 IFM 61572 IFM 63738 IFM 50669 IFM 63805 IFM 65036 IFM 59832 IFM 55044 IFM 64732 IFM 47670 IFM 51978 IFM 58328 IFM 54842 IFM 60369 IFM 65136 IFM 62804 IFM 57543 IFM 54229 (= Af293) IFM53842 (= A1163 = CEA10) IFM 65639 (= G54V)