RRC ID 58989
Author Traut W, Schubert V, Daliková M, Marec F, Sahara K.
Title Activity and inactivity of moth sex chromosomes in somatic and meiotic cells.
Journal Chromosoma
Abstract Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species.
Volume 128(4)
Pages 533-545
Published 2019-12
DOI 10.1007/s00412-019-00722-8
PII 10.1007/s00412-019-00722-8
PMID 31410566
MeSH Animals Bombyx / genetics Bombyx / metabolism Chromatin / metabolism* Chromosomes, Insect / metabolism Meiosis Moths / genetics* Moths / metabolism Sex Chromosomes / metabolism*
IF 3.53
Resource
Silkworms p50