Reference - Detail
| RRC ID | 58992 |
|---|---|
| Author | Kinoshita Y, Xu J, Masuda A, Minamihata K, Kamiya N, Mon H, Fujita R, Kusakabe T, Lee JM. |
| Title | Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system. |
| Journal | Protein Expr Purif |
| Abstract |
Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a hematopoietic growth factor. It is widely employed as a therapeutic agent targeting neutropenia in cancer patients undergoing chemotherapy and in patients with AIDS or after bone marrow transplantation. In this study, we constructed the recombinant baculoviruses for the expression of recombinant hGM-CSF (rhGM-CSF) with two small affinity tags (His-tag and Strep-tag) at the N or C-terminus. Compared to N-tagged rhGM-CSF, C-tagged rhGM-CSF was highly recovered from silkworm hemolymph. The purified rhGM-CSF proteins migrated as a diffuse band and were confirmed to hold N-glycosylations. A comparable activity was achieved when commercial hGM-CSF was tested as a control. Considering the high price of hGM-CSF in the market, our results and strategies using silkworm-baculovirus system can become a great reference for mass production of the active rhGM-CSF at a lower cost. |
| Volume | 159 |
| Pages | 69-74 |
| Published | 2019-7-1 |
| DOI | 10.1016/j.pep.2019.03.010 |
| PII | S1046-5928(19)30082-8 |
| PMID | 30917920 |
| MeSH | Animals Baculoviridae / genetics Base Sequence Bombyx / genetics Cell Extracts / chemistry Cell Line Chromatography, Affinity Gene Expression Glycosylation Granulocyte-Macrophage Colony-Stimulating Factor / chemistry* Granulocyte-Macrophage Colony-Stimulating Factor / genetics* Humans Hydrogen-Ion Concentration Protein Stability Recombinant Proteins / chemistry* Recombinant Proteins / genetics* Virus Diseases |
| IF | 1.513 |
| Times Cited | 4 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | n17 |