論文 - 詳細
| RRC ID | 590 |
|---|---|
| 著者 | Mitsuda N, Hisabori T, Takeyasu K, Sato MH. |
| タイトル | VOZ; isolation and characterization of novel vascular plant transcription factors with a one-zinc finger from Arabidopsis thaliana. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53% amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss, Physcomitrella patens. Promoter-beta-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family proteins and designate it as the VOZ-domain. |
| 巻・号 | 45(7) |
| ページ | 845-54 |
| 公開日 | 2004-7-1 |
| DOI | 10.1093/pcp/pch101 |
| PII | 45/7/845 |
| PMID | 15295067 |
| MeSH | Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Binding Sites / genetics DNA, Complementary / analysis DNA, Complementary / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / isolation & purification DNA-Binding Proteins / metabolism* Gene Expression Regulation, Plant / genetics Molecular Sequence Data Plant Roots / cytology Plant Roots / genetics Plant Roots / metabolism Protein Binding / genetics Sequence Homology, Nucleic Acid Transcription Factors / genetics Transcription Factors / isolation & purification Transcription Factors / metabolism* Transcriptional Activation / genetics Zinc Fingers / genetics |
| IF | 4.062 |
| 引用数 | 50 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdp24849 |