RRC ID 59030
Author Li Y, Yang H, Zhang H, Liu Y, Shang H, Zhao H, Zhang T, Tu Q.
Title Decode-seq: a practical approach to improve differential gene expression analysis.
Journal Genome Biol
Abstract Many differential gene expression analyses are conducted with an inadequate number of biological replicates. We describe an easy and effective RNA-seq approach using molecular barcoding to enable profiling of a large number of replicates simultaneously. This approach significantly improves the performance of differential gene expression analysis. Using this approach in medaka (Oryzias latipes), we discover novel genes with sexually dimorphic expression and genes necessary for germ cell development. Our results also demonstrate why the common practice of using only three replicates in differential gene expression analysis should be abandoned.
Volume 21(1)
Pages 66
Published 2020-3-23
DOI 10.1186/s13059-020-01966-9
PII 10.1186/s13059-020-01966-9
PMID 32200760
PMC PMC7087377
MeSH Animals Female Humans Male Mice Oryzias / genetics RNA-Seq / methods* Sex Determination Processes / genetics
IF 10.806
Times Cited 0
Medaka d-rR-Tg(olvas-GFP) (TG141) Qurt (MT828) hatching enzyme