Reference - Detail
| RRC ID | 59060 |
|---|---|
| Author | Jindal S, Thampy H, Day PJR, Kell DB. |
| Title | Very rapid flow cytometric assessment of antimicrobial susceptibility during the apparent lag phase of microbial (re)growth. |
| Journal | Microbiology (Reading) |
| Abstract |
Rapid changes in the number and flow cytometric behaviour of cells of E. coli taken from a stationary phase and inoculated into rich medium.Cells of E. coli were grown in LB medium, taken from a stationary phase of 2-4 h, and re-inoculated into fresh media at a concentration (105 ml-1 or lower) characteristic of bacteriuria. Flow cytometry was used to assess how quickly we could detect changes in cell size, number, membrane energization (using a carbocyanine dye) and DNA distribution. It transpired that while the lag phase observable macroscopically via bulk OD measurements could be as long as 4 h, the true lag phase could be less than 15-20 min, and was accompanied by many observable biochemical changes. Antibiotics to which the cells were sensitive affected these changes within 20 min of re-inoculation, providing the possibility of a very rapid antibiotic susceptibility test on a timescale compatible with a visit to a GP clinic. The strategy was applied successfully to genuine potential urinary tract infection (UTI) samples taken from a doctor's surgery. The methods developed could prove of considerable value in ensuring the correct prescription and thereby lowering the spread of antimicrobial resistance. |
| Volume | 165(4) |
| Pages | 439-454 |
| Published | 2019-4-1 |
| DOI | 10.1099/mic.0.000777 |
| PMID | 30741628 |
| MeSH | Anti-Bacterial Agents / pharmacology* Anti-Bacterial Agents / therapeutic use Bacteria / drug effects* Bacteria / growth & development* Bacteria / isolation & purification Bacteriuria / drug therapy Bacteriuria / microbiology Culture Media Flow Cytometry* Humans Microbial Sensitivity Tests / methods* Urinary Tract Infections / drug therapy Urinary Tract Infections / microbiology* |
| IF | 1.922 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ASKA (-) collection |