RRC ID 59062
著者 Warnhoff K, Ruvkun G.
タイトル Molybdenum cofactor transfer from bacteria to nematode mediates sulfite detoxification.
ジャーナル Nat Chem Biol
Abstract The kingdoms of life share many small molecule cofactors and coenzymes. Molybdenum cofactor (Moco) is synthesized by many archaea, bacteria, and eukaryotes, and is essential for human development. The genome of Caenorhabditis elegans contains all of the Moco biosynthesis genes, and surprisingly these genes are not essential if the animals are fed a bacterial diet that synthesizes Moco. C. elegans lacking both endogenous Moco synthesis and dietary Moco from bacteria arrest development, demonstrating interkingdom Moco transfer. Our screen of Escherichia coli mutants identifies genes necessary for synthesis of bacterial Moco or transfer to C. elegans. Developmental arrest of Moco-deficient C. elegans is caused by loss of sulfite oxidase, a Moco-requiring enzyme, and is suppressed by mutations in either C. elegans cystathionine gamma-lyase or cysteine dioxygenase, blocking toxic sulfite production from cystathionine. Thus, we define the genetic pathways for an interkingdom dialogue focused on sulfur homeostasis.
巻・号 15(5)
ページ 480-488
公開日 2019-5-1
DOI 10.1038/s41589-019-0249-y
PII 10.1038/s41589-019-0249-y
PMID 30911177
PMC PMC6470025
MeSH Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / metabolism* Coenzymes / metabolism* Homeostasis Metalloproteins / metabolism* Pteridines / metabolism* Sulfites / metabolism* Sulfites / toxicity
IF 12.154
引用数 3
リソース情報
原核生物(大腸菌) Keio collection