RRC ID 59088
著者 Miyake Y, Yamamoto K.
タイトル Epistatic Effect of Regulators to the Adaptive Growth of Escherichia coli.
ジャーナル Sci Rep
Abstract Bacteria survive in the environment with three steps: a sensing environmental conditions, a responding to sensed signals, and an adaptation for proper survival in the environment. An adapting bacterial cell occurs cell division to increase the number of sister cells, termed adaptive growth. Two-component systems (TCSs), representing the main bacterial signal transduction systems, consist of a pair of one sensor kinase (SK) and one response regulator (RR), and RR genes are abundant in most bacterial genomes as part of the core genome. The OmpR gene family, a group of RR genes, is conserved in 95% of known bacterial genomes. The Escherichia coli genome has an estimated 34 RR genes in total, including 14 genes of OmpR family genes. To reveal the contribution of TCSs for fast growth as an adaptive growth strategy of E. coli, we isolated a set of gene knockout strains by using newly developed genome editing technology, the HoSeI (Homologous Sequence Integration) method, based on CRISPR-Cas9. The statistics of single cell observation show a knockout of an arbitrary pair of phoP, phoB, and ompR genes, stably expressed by positive feedback regulation, dramatically inhibit the optimum adaptive growth of E. coli. These insights suggest that the adaptive growth of bacteria is fulfilled by the optimum high intracellular level of regulators acquired during growth under environmental conditions.
巻・号 10(1)
ページ 3661
公開日 2020-2-27
DOI 10.1038/s41598-020-60353-3
PII 10.1038/s41598-020-60353-3
PMID 32108145
PMC PMC7046781
MeSH Adaptation, Biological / genetics* Epistasis, Genetic* Escherichia coli* / genetics Escherichia coli* / growth & development Escherichia coli Proteins* / biosynthesis Escherichia coli Proteins* / genetics Gene Editing Gene Expression Regulation, Bacterial* Genome, Bacterial*
IF 4.011
リソース情報
原核生物(大腸菌) MG1655 psgRNA ME9903 ME9904 ME9905 ME9906 ME9907 ME9908 ME9919 ME9909 ME9920 ME9910 ME9921 ME9922 ME9911 ME9923 ME9924 ME9912 ME9925 ME9926 ME9913 ME9927 ME9914 ME9928 ME9929 ME9915 ME9930 ME9931 ME9916 ME9932 ME9933 ME9917 ME9934 ME9918 ME9935 psgRNA-ompR psgRNA-phoB psgRNA-phoP psgRNA-kdpE psgRNA-creB psgRNA-cpxR psgRNA-rstA psgRNA-hprR psgRNA-arcA psgRNA-basR psgRNA-baeR psgRNA-qseB psgRNA-torR psgRNA-cusR psgRNA-envZ psgRNA-phoR psgRNA-phoQ pLux-mgtA pLux-pstS pLux-ompC pBADPhoP-FLAG pBADPhoB-FLAG pBADOmpR-FLAG