RRC ID 59109
Author Ishimoto H, Kamikouchi A.
Title A Feedforward Circuit Regulates Action Selection of Pre-mating Courtship Behavior in Female Drosophila.
Journal Curr Biol
Abstract In the early phase of courtship, female fruit flies exhibit an acute rejection response to avoid unfavorable mating. This pre-mating rejection response is evolutionarily paralleled across species, but the molecular and neuronal basis of that behavior is unclear. Here, we show that a putative incoherent feedforward circuit comprising ellipsoid body neurons, cholinergic R4d, and its repressor GABAergic R2/R4m neurons regulates the pre-mating rejection response in the virgin female Drosophila melanogaster. Both R4d and R2/R4m are positively regulated, via specific dopamine receptors, by a subset of neurons in the dopaminergic PPM3 cluster. Genetic deprivation of GABAergic signal via GABAA receptor RNA interference in this circuit induces a massive rejection response, whereas activation of GABAergic R2/R4m or suppression of cholinergic R4d increases receptivity. Moreover, glutamatergic signaling via N-methyl-d-aspartate receptors induces NO-mediated retrograde regulation potentially from R4d to R2/R4m, likely providing flexible control of the behavioral switching from rejection to acceptance. Our study elucidates the molecular and neural mechanisms regulating the behavioral selection process of the pre-mating female.
Volume 30(3)
Pages 396-407.e4
Published 2020-2-3
DOI 10.1016/j.cub.2019.11.065
PII S0960-9822(19)31567-2
PMID 31902724
MeSH Animals Cell Communication Courtship Drosophila melanogaster / physiology* Female GABAergic Neurons / physiology Neurons / physiology* Sexual Behavior, Animal / physiology*
IF 9.601
Times Cited 2
Drosophila DGRC#103898