| RRC ID |
59121
|
| 著者 |
Das P, Salazar JL, Li-Kroeger D, Yamamoto S, Nakamura M, Sasamura T, Inaki M, Masuda W, Kitagawa M, Yamakawa T, Matsuno K.
|
| タイトル |
Maternal almondex, a neurogenic gene, is required for proper subcellular Notch distribution in early Drosophila embryogenesis.
|
| ジャーナル |
Dev Growth Differ
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| Abstract |
Notch signaling plays crucial roles in the control of cell fate and physiology through local cell-cell interactions. The core processes of Notch signal transduction are well established, but the mechanisms that fine-tune the pathway in various developmental and post-developmental contexts are less clear. Drosophila almondex, which encodes an evolutionarily conserved double-pass transmembrane protein, was identified in the 1970s as a maternal-effect gene that regulates Notch signaling in certain contexts, but its mechanistic function remains obscure. In this study, we examined the role of almondex in Notch signaling during early Drosophila embryogenesis. We found that in addition to being required for lateral inhibition in the neuroectoderm, almondex is also partially required for Notch signaling-dependent single-minded expression in the mesectoderm. Furthermore, we found that almondex is required for proper subcellular Notch receptor distribution in the neuroectoderm, specifically during mid-stage 5 development. The absence of maternal almondex during this critical window of time caused Notch to accumulate abnormally in cells in a mesh-like pattern. This phenotype did not include any obvious change in subcellular Delta ligand distribution, suggesting that it does not result from a general vesicular-trafficking defect. Considering that dynamic Notch trafficking regulates signal output to fit the specific context, we speculate that almondex may facilitate Notch activation by regulating intracellular Notch receptor distribution during early embryogenesis.
|
| 巻・号 |
62(1)
|
| ページ |
80-93
|
| 公開日 |
2020-1-1
|
| DOI |
10.1111/dgd.12639
|
| PMID |
31782145
|
| MeSH |
Animals
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster
Embryo, Nonmammalian / metabolism*
Embryonic Development*
Female
Neurogenesis*
Receptors, Notch / genetics
Receptors, Notch / metabolism*
Signal Transduction*
|
| IF |
1.723
|
| 引用数 |
1
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| リソース情報 |
| ショウジョウバエ |
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