論文 - 詳細
| RRC ID | 59134 |
|---|---|
| 著者 | Tsai CR, Galko MJ. |
| タイトル | Casein kinase 1α decreases β-catenin levels at adherens junctions to facilitate wound closure in Drosophila larvae. |
| ジャーナル | Development |
| Abstract |
Skin wound repair is essential to restore barrier function and prevent infection after tissue damage. Wound-edge epidermal cells migrate as a sheet to close the wound. However, it is still unclear how cell-cell junctions are regulated during wound closure (WC). To study this, we examined adherens junctions during WC in Drosophila larvae. β-Catenin is reduced at the lateral cell-cell junctions of wound-edge epidermal cells in the early healing stages. Destruction complex components, including Ck1α, GSK3β and β-TrCP, suppress β-catenin levels in the larval epidermis. Tissue-specific RNAi targeting these genes also caused severe WC defects. The Ck1αRNAi -induced WC defect is related to adherens junctions because loss of either β-catenin or E-cadherin significantly rescued this WC defect. In contrast, TCFRNAi does not rescue the Ck1αRNAi -induced WC defect, suggesting that Wnt signaling is not related to this defect. Direct overexpression of β-catenin recapitulates most of the features of Ck1α reduction during wounding. Finally, loss of Ck1α also blocked junctional E-cadherin reduction around the wound. Our results suggest that Ck1α and the destruction complex locally regulate cell adhesion to facilitate efficient wound repair. |
| 巻・号 | 146(20) |
| 公開日 | 2019-10-2 |
| DOI | 10.1242/dev.175133 |
| PII | dev.175133 |
| PMID | 31511254 |
| PMC | PMC6826034 |
| MeSH | Adherens Junctions / metabolism* Animals Cadherins / metabolism Casein Kinase Ialpha / metabolism* Drosophila Epidermal Cells / metabolism Epidermis / metabolism Glycogen Synthase Kinase 3 beta / metabolism Larva / metabolism* beta Catenin / metabolism* beta-Transducin Repeat-Containing Proteins / metabolism |
| IF | 5.611 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | GL00021 HMS02276 HMS00693 |