RRC ID 59147
Author Chen DY, Crest J, Streichan SJ, Bilder D.
Title Extracellular matrix stiffness cues junctional remodeling for 3D tissue elongation.
Journal Nat Commun
Abstract Organs are sculpted by extracellular as well as cell-intrinsic forces, but how collective cell dynamics are orchestrated in response to environmental cues is poorly understood. Here we apply advanced image analysis to reveal extracellular matrix-responsive cell behaviors that drive elongation of the Drosophila follicle, a model system in which basement membrane stiffness instructs three-dimensional tissue morphogenesis. Through in toto morphometric analyses of wild type and round egg mutants, we find that neither changes in average cell shape nor oriented cell division are required for appropriate organ shape. Instead, a major element is the reorientation of elongated cells at the follicle anterior. Polarized reorientation is regulated by mechanical cues from the basement membrane, which are transduced by the Src tyrosine kinase to alter junctional E-cadherin trafficking. This mechanosensitive cellular behavior represents a conserved mechanism that can elongate edgeless tubular epithelia in a process distinct from those that elongate bounded, planar epithelia.
Volume 10(1)
Pages 3339
Published 2019-7-26
DOI 10.1038/s41467-019-10874-x
PII 10.1038/s41467-019-10874-x
PMID 31350387
PMC PMC6659696
MeSH Animals Basement Membrane / chemistry Basement Membrane / growth & development Basement Membrane / metabolism Cadherins / genetics Cadherins / metabolism Cell Polarity Cell Shape Drosophila / chemistry Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Extracellular Matrix / chemistry* Extracellular Matrix / genetics Extracellular Matrix / metabolism Female Ovarian Follicle / growth & development* Ovarian Follicle / metabolism
IF 12.121
Times Cited 5
Resource
Drosophila DGRC#104055