論文 - 詳細
| RRC ID | 59150 |
|---|---|
| 著者 | Zheng Y, Buchwalter RA, Zheng C, Wight EM, Chen JV, Megraw TL. |
| タイトル | A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Non-centrosomal microtubule-organizing centres (ncMTOCs) have a variety of roles that are presumed to serve the diverse functions of the range of cell types in which they are found. ncMTOCs are diverse in their composition, subcellular localization and function. Here we report a perinuclear MTOC in Drosophila fat body cells that is anchored by the Nesprin homologue Msp300 at the cytoplasmic surface of the nucleus. Msp300 recruits the microtubule minus-end protein Patronin, a calmodulin-regulated spectrin-associated protein (CAMSAP) homologue, which functions redundantly with Ninein to further recruit the microtubule polymerase Msps-a member of the XMAP215 family-to assemble non-centrosomal microtubules and does so independently of the widespread microtubule nucleation factor γ-Tubulin. Functionally, the fat body ncMTOC and the radial microtubule arrays that it organizes are essential for nuclear positioning and for secretion of basement membrane components via retrograde dynein-dependent endosomal trafficking that restricts plasma membrane growth. Together, this study identifies a perinuclear ncMTOC with unique architecture that regulates microtubules, serving vital functions. |
| 巻・号 | 22(3) |
| ページ | 297-309 |
| 公開日 | 2020-3-1 |
| DOI | 10.1038/s41556-020-0470-7 |
| PII | 10.1038/s41556-020-0470-7 |
| PMID | 32066907 |
| PMC | PMC7161059 |
| MeSH | Actins / physiology Animals Basement Membrane / metabolism* Cell Membrane Cell Nucleus* / ultrastructure Centrosome Drosophila / metabolism Drosophila / ultrastructure Drosophila Proteins / metabolism Drosophila Proteins / physiology Dyneins / physiology Endosomes / metabolism Fat Body / metabolism Fat Body / ultrastructure Membrane Proteins / metabolism Microfilament Proteins / metabolism Microfilament Proteins / physiology Microtubule-Associated Proteins / metabolism Microtubule-Associated Proteins / physiology Microtubule-Organizing Center / physiology* Microtubule-Organizing Center / ultrastructure Microtubules / physiology Muscle Proteins / metabolism Tubulin / physiology |
| IF | 20.042 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 24 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#110692 DGRC#115262 |