論文 - 詳細
| RRC ID | 59155 |
|---|---|
| 著者 | He L, Binari R, Huang J, Falo-Sanjuan J, Perrimon N. |
| タイトル | In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer. |
| ジャーナル | Elife |
| Abstract |
Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers to monitor pathway activities and determine cell type identities. However, a large amount of dynamic information is lost due to the long half-life of the fluorescent proteins. To better detect dynamics, fluorescent transcriptional reporters can be destabilized to shorten their half-lives. However, applications of this approach in vivo are limited due to significant reduction of signal intensities. To overcome this limitation, we enhanced translation of a destabilized fluorescent protein and demonstrate the advantages of this approach by characterizing spatio-temporal changes of transcriptional activities in Drosophila. In addition, by combining a fast-folding destabilized fluorescent protein and a slow-folding long-lived fluorescent protein, we generated a dual-color transcriptional timer that provides spatio-temporal information about signaling pathway activities. Finally, we demonstrate the use of this transcriptional timer to identify new genes with dynamic expression patterns. |
| 巻・号 | 8 |
| 公開日 | 2019-5-29 |
| DOI | 10.7554/eLife.46181 |
| PII | 46181 |
| PMID | 31140975 |
| PMC | PMC6660218 |
| MeSH | Animals Drosophila melanogaster / embryology Drosophila melanogaster / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Enhancer Elements, Genetic / genetics Fluorescence Gene Expression Regulation* Green Fluorescent Proteins / metabolism Intestines / cytology Protein Biosynthesis Receptors, Notch / metabolism STAT Transcription Factors / metabolism Stem Cells / cytology Transcription, Genetic* |
| IF | 7.08 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 58 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#112553 |