RRC ID |
59166
|
著者 |
Koyama LAJ, Aranda-Díaz A, Su YH, Balachandra S, Martin JL, Ludington WB, Huang KC, O'Brien LE.
|
タイトル |
Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.
|
ジャーナル |
PLoS Biol
|
Abstract |
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
|
巻・号 |
18(1)
|
ページ |
e3000567
|
公開日 |
2020-1-1
|
DOI |
10.1371/journal.pbio.3000567
|
PII |
PBIOLOGY-D-19-02718
|
PMID |
31986129
|
PMC |
PMC7004386
|
MeSH |
Age Factors
Anatomy, Cross-Sectional / methods*
Animals
Drosophila / anatomy & histology*
Drosophila / microbiology
Gastrointestinal Microbiome*
Intestines / anatomy & histology
Intestines / diagnostic imaging
Intravital Microscopy / methods*
Optical Imaging / methods
Viscera / anatomy & histology*
Viscera / diagnostic imaging
|
IF |
7.076
|
引用数 |
0
|
リソース情報 |
ショウジョウバエ |
DGRC#112304 |