RRC ID 59166
Author Koyama LAJ, Aranda-Díaz A, Su YH, Balachandra S, Martin JL, Ludington WB, Huang KC, O'Brien LE.
Title Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.
Journal PLoS Biol
Abstract Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
Volume 18(1)
Pages e3000567
Published 2020-1-1
DOI 10.1371/journal.pbio.3000567
PII PBIOLOGY-D-19-02718
PMID 31986129
PMC PMC7004386
MeSH Age Factors Anatomy, Cross-Sectional / methods* Animals Drosophila / anatomy & histology* Drosophila / microbiology Gastrointestinal Microbiome* Intestines / anatomy & histology Intestines / diagnostic imaging Intravital Microscopy / methods* Optical Imaging / methods Viscera / anatomy & histology* Viscera / diagnostic imaging
IF 7.076
Times Cited 0
Resource
Drosophila DGRC#112304