RRC ID 59170
著者 Sohr A, Du L, Wang R, Lin L, Roy S.
タイトル Drosophila FGF cleavage is required for efficient intracellular sorting and intercellular dispersal.
ジャーナル J Cell Biol
Abstract How morphogenetic signals are prepared for intercellular dispersal and signaling is fundamental to the understanding of tissue morphogenesis. We discovered an intracellular mechanism that prepares Drosophila melanogaster FGF Branchless (Bnl) for cytoneme-mediated intercellular dispersal during the development of the larval Air-Sac-Primordium (ASP). Wing-disc cells express Bnl as a proprotein that is cleaved by Furin1 in the Golgi. Truncated Bnl sorts asymmetrically to the basal surface, where it is received by cytonemes that extend from the recipient ASP cells. Uncleavable mutant Bnl has signaling activity but is mistargeted to the apical side, reducing its bioavailability. Since Bnl signaling levels feedback control cytoneme production in the ASP, the reduced availability of mutant Bnl on the source basal surface decreases ASP cytoneme numbers, leading to a reduced range of signal/signaling gradient and impaired ASP growth. Thus, enzymatic cleavage ensures polarized intracellular sorting and availability of Bnl to its signaling site, thereby determining its tissue-specific intercellular dispersal and signaling range.
巻・号 218(5)
ページ 1653-1669
公開日 2019-5-6
DOI 10.1083/jcb.201810138
PII jcb.201810138
PMID 30808704
PMC PMC6504889
MeSH Air Sacs / cytology Air Sacs / metabolism* Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / growth & development Animals, Genetically Modified / metabolism* Cell Movement Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Fibroblast Growth Factors / genetics Fibroblast Growth Factors / metabolism* Furin / genetics Furin / metabolism Imaginal Discs / cytology Imaginal Discs / metabolism* Protein Transport Wings, Animal / cytology Wings, Animal / metabolism*
IF 8.811
引用数 3
リソース情報
ショウジョウバエ DGRC#104593