RRC ID 59183
Author Kirkland NJ, Yuen AC, Tozluoglu M, Hui N, Paluch EK, Mao Y.
Title Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development.
Journal Curr Biol
Abstract Cell divisions are essential for tissue growth. In pseudostratified epithelia, where nuclei are staggered across the tissue, each nucleus migrates apically before undergoing mitosis. Successful apical nuclear migration is critical for planar-orientated cell divisions in densely packed epithelia. Most previous investigations have focused on the local cellular mechanisms controlling nuclear migration. Inter-species and inter-organ comparisons of different pseudostratified epithelia suggest global tissue architecture may influence nuclear dynamics, but the underlying mechanisms remain elusive. Here, we use the developing Drosophila wing disc to systematically investigate, in a single epithelial type, how changes in tissue architecture during growth influence mitotic nuclear migration. We observe distinct nuclear dynamics at discrete developmental stages, as epithelial morphology changes. We use genetic and physical perturbations to show a direct effect of cell density on mitotic nuclear positioning. We find Rho kinase and Diaphanous, which facilitate mitotic cell rounding in confined cell conditions, are essential for efficient apical nuclear movement. Perturbation of Diaphanous causes increasing defects in apical nuclear migration as the tissue grows and cell density increases, and these defects can be reversed by acute physical reduction of cell density. Our findings reveal how the mechanical environment imposed on cells within a tissue alters the molecular and cellular mechanisms adopted by single cells for mitosis.
Volume 30(13)
Pages 2419-2432.e4
Published 2020-7-6
DOI 10.1016/j.cub.2020.04.041
PII S0960-9822(20)30555-8
PMID 32413305
PMC PMC7342018
MeSH Animals Cell Nucleus / metabolism* Drosophila melanogaster / growth & development Drosophila melanogaster / physiology* Epithelial Cells / physiology Epithelium / growth & development* Female Larva / growth & development Larva / physiology Male Mitosis*
IF 9.601
Times Cited 0
Drosophila DGRC#201233 DGRC#115366