Reference - Detail
| RRC ID | 59196 |
|---|---|
| Author | Nolivos S, Cayron J, Dedieu A, Page A, Delolme F, Lesterlin C. |
| Title | Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer. |
| Journal | Science |
| Abstract |
Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor-conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action. |
| Volume | 364(6442) |
| Pages | 778-782 |
| Published | 2019-5-24 |
| DOI | 10.1126/science.aav6390 |
| PII | 364/6442/778 |
| PMID | 31123134 |
| MeSH | Anti-Bacterial Agents / pharmacology Antiporters / antagonists & inhibitors Antiporters / biosynthesis Antiporters / genetics Bacterial Proteins / antagonists & inhibitors Bacterial Proteins / biosynthesis Bacterial Proteins / genetics Carrier Proteins / genetics Carrier Proteins / physiology* Conjugation, Genetic DNA, Single-Stranded Drug Resistance, Multiple, Bacterial / genetics* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / physiology* F Factor / genetics F Factor / physiology* Microscopy Protein Biosynthesis / drug effects Tetracycline / pharmacology |
| IF | 41.063 |
| Times Cited | 15 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 173 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME9806 |