論文 - 詳細
| RRC ID | 59230 |
|---|---|
| 著者 | Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI. |
| タイトル | Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The mechanisms that regulate germinal center (GC) B cell responses in the spleen are not fully understood. Here we use a combination of pharmacologic and genetic approaches to delete SIGN-R1+ marginal zone (MZ) macrophages and reveal their specific contribution to the regulation of humoral immunity in the spleen. We find that while SIGN-R1+ macrophages were not essential for initial activation of B cells, they were required for maturation of the response and development of GC B cells. These defects could be corrected when follicular helper T (Tfh) cells were induced before macrophage ablation or when Tfh responses were enhanced. Moreover, we show that in the absence of SIGN-R1+ macrophages, DCIR2+ dendritic cells (DCs), which play a key role in priming Tfh responses, were unable to cluster to the interfollicular regions of the spleen and were instead displaced to the MZ. Restoring SIGN-R1+ macrophages to the spleen corrected positioning of DCIR2+ DCs and rescued the GC B cell response. Our study reveals a previously unappreciated role for SIGN-R1+ macrophages in regulation of the GC reaction and highlights the functional specification of macrophage subsets in the MZ compartment. |
| 巻・号 | 117(22) |
| ページ | 12295-12305 |
| 公開日 | 2020-6-2 |
| DOI | 10.1073/pnas.1921673117 |
| PII | 1921673117 |
| PMID | 32424104 |
| PMC | PMC7275705 |
| MeSH | Animals B-Lymphocytes / immunology* Cell Adhesion Molecules / genetics Cell Adhesion Molecules / immunology* Germinal Center / immunology* Lectins, C-Type / genetics Lectins, C-Type / immunology* Lymphocyte Activation Macrophages / immunology* Mice, Inbred C57BL Mice, Knockout Receptors, Cell Surface / genetics Receptors, Cell Surface / immunology* Spleen / immunology* T-Lymphocytes, Helper-Inducer |
| IF | 9.412 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC04395 |