| RRC ID |
59272
|
| Author |
Karpf S, Riche CT, Di Carlo D, Goel A, Zeiger WA, Suresh A, Portera-Cailliau C, Jalali B.
|
| Title |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.
|
| Journal |
Nat Commun
|
| Abstract |
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.
|
| Volume |
11(1)
|
| Pages |
2062
|
| Published |
2020-4-28
|
| DOI |
10.1038/s41467-020-15618-w
|
| PII |
10.1038/s41467-020-15618-w
|
| PMID |
32346060
|
| PMC |
PMC7188897
|
| MeSH |
Euglena / cytology
Imaging, Three-Dimensional
Microscopy, Fluorescence, Multiphoton*
Time Factors
|
| IF |
11.878
|
| Times Cited |
1
|
| Resource |
| Algae |
NIES-48 |
