Reference - Detail
| RRC ID | 59272 |
|---|---|
| Author | Karpf S, Riche CT, Di Carlo D, Goel A, Zeiger WA, Suresh A, Portera-Cailliau C, Jalali B. |
| Title | Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates. |
| Journal | Nat Commun |
| Abstract |
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting. |
| Volume | 11(1) |
| Pages | 2062 |
| Published | 2020-4-28 |
| DOI | 10.1038/s41467-020-15618-w |
| PII | 10.1038/s41467-020-15618-w |
| PMID | 32346060 |
| PMC | PMC7188897 |
| MeSH | Euglena / cytology Imaging, Three-Dimensional Microscopy, Fluorescence, Multiphoton* Time Factors |
| IF | 11.878 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-48 |