RRC ID 59295
Author Zhang Y, Hu L, Qiu Y, Osei-Adjei G, Tang H, Zhang Y, Zhang R, Sheng X, Xu S, Yang W, Yang H, Yin Z, Yang R, Huang X, Zhou D.
Title QsvR integrates into quorum sensing circuit to control Vibrio parahaemolyticus virulence.
Journal Environ. Microbiol.
Abstract Vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis worldwide, requires the two type-III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) for full virulence. The tdh genes and the T3SS2 gene cluster constitute an 80 kb pathogenicity island known as Vp-PAI located on the chromosome II. Expression of T3SS1 and Vp-PAI is regulated in a quorum sensing (QS)-dependent manner but its detailed mechanisms remain unknown. Herein, we show that three factors (QS regulators AphA and OpaR and an AraC-type transcriptional regulator QsvR) form a complex regulatory network to control the expression of T3SS1 and Vp-PAI genes. At low cell density (LCD), whereas Vp-PAI expression is repressed, T3SS1 genes are induced by AphA, which directly binds (an operator region of) the exsBAD-vscBCD operon. At high cell density (HCD), the bacterium turns off T3SS1 expression by replacing AphA with OpaR, triggering the induction of Vp-PAI. Furthermore, QsvR binds to the regulatory regions of all the tested T3SS1 and Vp-PAI genes to activate their transcription at HCD. Taken together, our data highlight how multiple QS regulators contribute to the pathogenicity of V. parahaemolyticus by precisely controlling the expression of major virulence determinants during different stages of growth.
Volume 21(3)
Pages 1054-1067
Published 2019-3
DOI 10.1111/1462-2920.14524
PMID 30623553
MeSH Animals Bacterial Proteins / physiology Bacterial Toxins / genetics Female Gene Expression Regulation, Bacterial* Hemolysin Proteins / genetics Humans Mice Mice, Inbred BALB C Multigene Family Operon Quorum Sensing* / genetics Rabbits Vibrio Infections / microbiology Vibrio parahaemolyticus / genetics Vibrio parahaemolyticus / pathogenicity* Virulence Virulence Factors / genetics Virulence Factors / metabolism
IF 5.147
Resource
Pathogenic bacteria Vibrio parahaemolyticus RIMD2210633