RRC ID 5931
Author Miyahara M, Kim SW, Fushinobu S, Takaki K, Yamada T, Watanabe A, Miyauchi K, Endo G, Wakagi T, Shoun H.
Title Potential of aerobic denitrification by Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment plants.
Journal Appl. Environ. Microbiol.
Abstract In contrast to most denitrifiers studied so far, Pseudomonas stutzeri TR2 produces low levels of nitrous oxide (N(2)O) even under aerobic conditions. We compared the denitrification activity of strain TR2 with those of various denitrifiers in an artificial medium that was derived from piggery wastewater. Strain TR2 exhibited strong denitrification activity and produced little N(2)O under all conditions tested. Its growth rate under denitrifying conditions was near comparable to that under aerobic conditions, showing a sharp contrast to the lower growth rates of other denitrifiers under denitrifying conditions. Strain TR2 was tolerant to toxic nitrite, even utilizing it as a good denitrification substrate. When both nitrite and N(2)O were present, strain TR2 reduced N(2)O in preference to nitrite as the denitrification substrate. This bacterial strain was readily able to adapt to denitrifying conditions by expressing the denitrification genes for cytochrome cd(1) nitrite reductase (NiR) (nirS) and nitrous oxide reductase (NoS) (nosZ). Interestingly, nosZ was constitutively expressed even under nondenitrifying, aerobic conditions, consistent with our finding that strain TR2 preferred N(2)O to nitrite. These properties of strain TR2 concerning denitrification are in sharp contrast to those of well-characterized denitrifiers. These results demonstrate that some bacterial species, such as strain TR2, have adopted a strategy for survival by preferring denitrification to oxygen respiration. The bacterium was also shown to contain the potential to reduce N(2)O emissions when applied to sewage disposal fields.
Volume 76(14)
Pages 4619-25
Published 2010-7
DOI 10.1128/AEM.01983-09
PII AEM.01983-09
PMID 20495048
PMC PMC2901746
MeSH Aerobiosis Bacterial Proteins / biosynthesis Culture Media / chemistry Gene Expression Nitrite Reductases / biosynthesis Nitrites / metabolism* Nitrogen / metabolism* Nitrous Oxide / metabolism* Oxidoreductases / biosynthesis Pseudomonas stutzeri / growth & development Pseudomonas stutzeri / metabolism* Water Purification / methods*
IF 3.633
Times Cited 51
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
General Microbes JCM 2412 JCM 20620