| RRC ID |
59320
|
| 著者 |
Bowitch A, Michaels KL, Yu MC, Ferkey DM.
|
| タイトル |
The Protein Arginine Methyltransferase PRMT-5 Regulates SER-2 Tyramine Receptor-Mediated Behaviors in Caenorhabditis elegans.
|
| ジャーナル |
G3 (Bethesda)
|
| Abstract |
G protein-coupled receptors are 7-pass transmembrane receptors that couple to heterotrimeric G proteins to mediate cellular responses to a diverse array of stimuli. Understanding the mechanisms that regulate G protein-coupled receptors is crucial to manipulating their signaling for therapeutic benefit. One key regulatory mechanism that contributes to the functional diversity of many signaling proteins is post-translational modification. Whereas phosphorylation remains the best studied of such modifications, arginine methylation by protein arginine methyltransferases is emerging as a key regulator of protein function. We previously published the first functional evidence that arginine methylation of G protein-coupled receptors modulates their signaling. We report here a third receptor that is regulated by arginine methylation, the Caenorhabditis elegans SER-2 tyramine receptor. We show that arginines within a putative methylation motif in the third intracellular loop of SER-2 are methylated by PRMT5 in vitro Our data also suggest that this modification enhances SER-2 signaling in vivo to modulate animal behavior. The identification of a third G protein-coupled receptor to be functionally regulated by arginine methylation suggests that this post-translational modification may be utilized to regulate signaling through a broad array of G protein-coupled receptors.
|
| 巻・号 |
8(7)
|
| ページ |
2389-2398
|
| 公開日 |
2018-7-2
|
| DOI |
10.1534/g3.118.200360
|
| PII |
g3.118.200360
|
| PMID |
29760200
|
| PMC |
PMC6027898
|
| MeSH |
Animals
Animals, Genetically Modified
Arginine
Behavior, Animal*
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism*
Humans
Locomotion / genetics
Methylation
Protein-Arginine N-Methyltransferases / genetics*
Protein-Arginine N-Methyltransferases / metabolism*
Receptors, Biogenic Amine / metabolism*
Signal Transduction
|
| IF |
2.63
|
| 引用数 |
3
|
| リソース情報 |
| 線虫 |
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