論文 - 詳細
| RRC ID | 59320 |
|---|---|
| 著者 | Bowitch A, Michaels KL, Yu MC, Ferkey DM. |
| タイトル | The Protein Arginine Methyltransferase PRMT-5 Regulates SER-2 Tyramine Receptor-Mediated Behaviors in Caenorhabditis elegans. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
G protein-coupled receptors are 7-pass transmembrane receptors that couple to heterotrimeric G proteins to mediate cellular responses to a diverse array of stimuli. Understanding the mechanisms that regulate G protein-coupled receptors is crucial to manipulating their signaling for therapeutic benefit. One key regulatory mechanism that contributes to the functional diversity of many signaling proteins is post-translational modification. Whereas phosphorylation remains the best studied of such modifications, arginine methylation by protein arginine methyltransferases is emerging as a key regulator of protein function. We previously published the first functional evidence that arginine methylation of G protein-coupled receptors modulates their signaling. We report here a third receptor that is regulated by arginine methylation, the Caenorhabditis elegans SER-2 tyramine receptor. We show that arginines within a putative methylation motif in the third intracellular loop of SER-2 are methylated by PRMT5 in vitro Our data also suggest that this modification enhances SER-2 signaling in vivo to modulate animal behavior. The identification of a third G protein-coupled receptor to be functionally regulated by arginine methylation suggests that this post-translational modification may be utilized to regulate signaling through a broad array of G protein-coupled receptors. |
| 巻・号 | 8(7) |
| ページ | 2389-2398 |
| 公開日 | 2018-7-2 |
| DOI | 10.1534/g3.118.200360 |
| PII | g3.118.200360 |
| PMID | 29760200 |
| PMC | PMC6027898 |
| MeSH | Animals Animals, Genetically Modified Arginine Behavior, Animal* Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Humans Locomotion / genetics Methylation Protein-Arginine N-Methyltransferases / genetics* Protein-Arginine N-Methyltransferases / metabolism* Receptors, Biogenic Amine / metabolism* Signal Transduction |
| IF | 2.63 |
| 引用数 | 3 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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