論文 - 詳細
RRC ID | 5933 |
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著者 | Tsuji H, Moriyama K, Nomoto K, Miyanaga N, Akaza H. |
タイトル | Isolation and characterization of the equol-producing bacterium Slackia sp. strain NATTS. |
ジャーナル | Arch Microbiol |
Abstract |
Several kinds of carbohydrates such as sorbose, adonitol, and melezitose were found to enhance equol production from daidzein in an in vitro human fecal culture. Sorbose, one of the most effective carbohydrates, was used as a carbohydrate source for isolating the NATTS strain, which was a Gram-positive, non-spore-forming rod bacterium with high ability to convert daidzein to equol isolated from the 7th maintenance culture. The strain was found to belong to the genus Slackia family Coriobacteriaceae by 16S rRNA sequence-based analysis, and the prevalence of the Slackia sp. in Japanese adults was examined by reverse transcription-quantitative PCR (RT-qPCR), which was found to be 40% at a mean population level of 10(6) cells per gram of feces. |
巻・号 | 192(4) |
ページ | 279-87 |
公開日 | 2010-4-1 |
DOI | 10.1007/s00203-010-0546-z |
PMID | 20237913 |
MeSH | Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Actinobacteria / metabolism Adult Aged Carbohydrate Metabolism Culture Media DNA, Bacterial / genetics Equol Feces / microbiology Female Humans Isoflavones / biosynthesis* Isoflavones / metabolism Male Middle Aged Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Young Adult |
IF | 1.884 |
引用数 | 48 |
WOS 分野 | MICROBIOLOGY |
リソース情報 | |
一般微生物 | JCM 9981 JCM 1118 JCM 10300 JCM 10299 JCM 10643 JCM 10641 JCM 14552 JCM 14551 JCM 14553 JCM 12494 JCM 11022 JCM 14555 JCM 14554 JCM 5826 JCM 1290 JCM 5963 |