RRC ID 59344
Author Flatt KM, Beshers C, Unal C, Cohen JD, Sundaram MV, Schroeder NE.
Title Epidermal Remodeling in Caenorhabditis elegans Dauers Requires the Nidogen Domain Protein DEX-1.
Journal Genetics
Abstract Phenotypic plasticity is a critical component of an organism's ability to thrive in a changing environment. The free-living nematode Caenorhabditis elegans adapts to unfavorable environmental conditions by pausing reproductive development and entering a stress-resistant larval stage known as dauer. The transition into dauer is marked by vast morphological changes, including remodeling of epidermis, neurons, and muscle. Although many of these dauer-specific traits have been described, the molecular basis of dauer-specific remodeling is still poorly understood. Here we show that the nidogen domain-containing protein DEX-1 facilitates stage-specific tissue remodeling during dauer morphogenesis. DEX-1 was previously shown to regulate sensory dendrite formation during embryogenesis. We find that DEX-1 is also required for proper remodeling of the stem cell-like epidermal seam cells. dex-1 mutant dauers lack distinct lateral cuticular alae during dauer and have increased sensitivity to sodium dodecyl sulfate. Furthermore, we find that DEX-1 is required for proper dauer mobility. We show that DEX-1 is secreted from the seam cells during dauer, but acts locally in a cell-autonomous manner. We find that dex-1 expression during dauer is regulated through DAF-16/FOXO-mediated transcriptional activation. Finally, we show that dex-1 acts with a family of zona pellucida domain-encoding genes to regulate dauer-specific epidermal remodeling. Taken together, our data indicate that DEX-1 is an extracellular matrix component that plays a central role in C. elegans epidermal remodeling during dauer.
Volume 211(1)
Pages 169-183
Published 2019-1-1
DOI 10.1534/genetics.118.301557
PII genetics.118.301557
PMID 30409788
PMC PMC6325711
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Epidermis / growth & development* Epidermis / metabolism Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism Larva / genetics Larva / growth & development Morphogenesis Stem Cells / cytology Stem Cells / metabolism
IF 3.564
Times Cited 2
Resource
C.elegans tm1126