論文 - 詳細
| RRC ID | 59355 |
|---|---|
| 著者 | He CW, Liao CP, Chen CK, Teulière J, Chen CH, Pan CL. |
| タイトル | The polarity protein VANG-1 antagonizes Wnt signaling by facilitating Frizzled endocytosis. |
| ジャーナル | Development |
| Abstract |
Signaling that instructs the migration of neurons needs to be tightly regulated to ensure precise positioning of neurons and subsequent wiring of the neuronal circuits. Wnt-Frizzled signaling controls neuronal migration in metazoans, in addition to many other aspects of neural development. We show that Caenorhabditis elegans VANG-1, a membrane protein that acts in the planar cell polarity (PCP) pathway, antagonizes Wnt signaling by facilitating endocytosis of the Frizzled receptors. Mutations of vang-1 suppress migration defects of multiple classes of neurons in the Frizzled mutants, and overexpression of vang-1 causes neuronal migration defects similar to those of the Frizzled mutants. Our genetic experiments suggest that VANG-1 facilitates Frizzled endocytosis through β-arrestin2. Co-immunoprecipitation experiments indicate that Frizzled proteins and VANG-1 form a complex, and this physical interaction requires the Frizzled cysteine-rich domain. Our work reveals a novel mechanism mediated by the PCP protein VANG-1 that downregulates Wnt signaling through Frizzled endocytosis. |
| 巻・号 | 145(24) |
| 公開日 | 2018-12-17 |
| DOI | 10.1242/dev.168666 |
| PII | dev.168666 |
| PMID | 30504124 |
| PMC | PMC6919297 |
| MeSH | Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Movement Cell Polarity* Endocytosis* Frizzled Receptors / metabolism* Larva / cytology Multiprotein Complexes / metabolism Neurons / cytology Neurons / metabolism Phosphoproteins / chemistry Phosphoproteins / genetics Phosphoproteins / metabolism* Wnt Signaling Pathway* |
| IF | 5.763 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1422 |